Team:Aachen/Project/2D Biosensor
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In the course of our project, we designed a casting mold specifically for the production of our agar chips. When the agar has cooled down, the chips are cut out of the mold and are ready to use. Storage of the readily usable sensor chips is possible for up to 2 days at 4 °C when using LB medium or up to 5 days if TB medium is used. A detailed description of the sensor chip manufacturing can be found in our [https://2014.igem.org/Team:Aachen/Notebook/Protocols/detection Protocols] section. | In the course of our project, we designed a casting mold specifically for the production of our agar chips. When the agar has cooled down, the chips are cut out of the mold and are ready to use. Storage of the readily usable sensor chips is possible for up to 2 days at 4 °C when using LB medium or up to 5 days if TB medium is used. A detailed description of the sensor chip manufacturing can be found in our [https://2014.igem.org/Team:Aachen/Notebook/Protocols/detection Protocols] section. | ||
- | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title= | + | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Assay to detect ''P. aeruginosa'' using ''Cellock Holmes''.|subtitle=This flow sheet shows the application of our 2D biosensor system.|width=1000px}} |
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{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=Mode of action inside ''WatsOn''.|subtitle=Chips are incubated and then illuminated with blue light. A picture is taken and analyzed for fluorescence signals using the software ''Measurarty''.|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=Mode of action inside ''WatsOn''.|subtitle=Chips are incubated and then illuminated with blue light. A picture is taken and analyzed for fluorescence signals using the software ''Measurarty''.|width=1000px}} | ||
- | Inside ''WatsOn'', the chips are incubated at 37 °C and populations of microorganisms | + | Inside ''WatsOn'', the chips are incubated at 37 °C and the sampled populations of microorganisms attached on the sampling chip start to grow and multiply. During incubation the chips can be '''illuminated with blue light''' at any time, and a '''photo of the chips''' is taken. The '''software ''Measurarty''''' then analyzes any fluorescent signal. ''P. aeruginosa'' secrets an increasing number of quorum sensing molecules that are recognized by ''Cellocks'' thereby producing a fluorescence signal. In case of ''P. aeruginosa'', we focused on a quorum sensing molecule called N-3-oxo-dodecanoyl-L-homoserine lactone (in short: 3-oxo-C<sub>12</sub>-HSL). The incorporation of 3-oxo-C<sub>12</sub>-HSL detection system into the sensor cells is explained in detail in the [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh Construct] section. |
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Revision as of 20:08, 16 October 2014
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