Team:Aachen/Project/2D Biosensor
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Further experiments were conducted concerining long-time storage of the sensor chips. Storage at -20 °C resulted in the loss of our sensor cells. Adding 5-10% glycerol ensured survival of the sensor cells, but resulted in an expression stop of fluorescence proteins. Thus the idea of long time storage of the sensor chips hab to be rejected. However, it was possible to store readily usable sensor chips for 2 days at 4 °C when using LB medium and storage for 5 days was possible, if TB medium was used. | Further experiments were conducted concerining long-time storage of the sensor chips. Storage at -20 °C resulted in the loss of our sensor cells. Adding 5-10% glycerol ensured survival of the sensor cells, but resulted in an expression stop of fluorescence proteins. Thus the idea of long time storage of the sensor chips hab to be rejected. However, it was possible to store readily usable sensor chips for 2 days at 4 °C when using LB medium and storage for 5 days was possible, if TB medium was used. | ||
- | In our own device ''WatsOn'' optimized wavelengths of 450 nm and 480 nm were used for excitation of iLOV and GFP, respectively. When exposed to both excitation wavelength the LB medium showed minimal background fluorescence and no difficulties were observed in cultivation of our sensor cells. Further reduction of background fluorescence compared to LB medium was observed when using TB medium for sensor chip | + | In our own device ''WatsOn'' optimized wavelengths of 450 nm and 480 nm were used for excitation of iLOV and GFP, respectively. When exposed to both excitation wavelength the LB medium showed minimal background fluorescence and no difficulties were observed in cultivation of our sensor cells. Further reduction of background fluorescence compared to LB medium was observed when using TB medium for sensor chip manufacturing in combination with fluorescence evaluation using ''WatsOn''. |
(5Tage_K131026_neb_tb_1,5h) | (5Tage_K131026_neb_tb_1,5h) | ||
Revision as of 19:26, 15 October 2014
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