Team:Aachen/Project/2D Biosensor
From 2014.igem.org
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(iLOV_GFP_HM_1,5h.png) | (iLOV_GFP_HM_1,5h.png) | ||
- | Concerning the medium used for our sensor chips, LB medium showed a high background fluorescence when exposed to UV light. Surprinsingly the background fluorescence resulting from the LB medium was to high to detect a signal emitted by our sensor cells, thus we used minimal media (NA, M9, Hartman) in order to minimize | + | Concerning the medium used for our sensor chips, LB medium showed a high background fluorescence when exposed to UV light. Surprinsingly the background fluorescence resulting from the LB medium was to high to detect a signal emitted by our sensor cells, thus we used minimal media (NA, M9, Hartman) in order to minimize background fluorescence. The appliction of minimal media was sufficient to minimize the background fluorescence, but this approach came with the drawback of minimal to zero growth of our sensor cells. |
(Chip_medium_geldoc.png) | (Chip_medium_geldoc.png) | ||
- | Further experiments were conducted concerining long-time storage of the sensor chips. Storage at -20 °C resulted in the loss of our sensor cells. Adding 5-10% glycerol ensured survival of the sensor cells, but resulted in an expression stop of fluorescence proteins. Thus the idea of long time storage of the sensor chips hab to be rejected. However, | + | Further experiments were conducted concerining long-time storage of the sensor chips. Storage at -20 °C resulted in the loss of our sensor cells. Adding 5-10% glycerol ensured survival of the sensor cells, but resulted in an expression stop of fluorescence proteins. Thus the idea of long time storage of the sensor chips hab to be rejected. However, it was possible to store readily usable sensor chips for 2 days at 4 °C when using LB medium and storage for 5 days was possible, if TB medium was used. |
In our own device WatsOn with the optimized light (450/ 480 nm) the LB showed no fluorescence. The cells grow better there and so we use this now. | In our own device WatsOn with the optimized light (450/ 480 nm) the LB showed no fluorescence. The cells grow better there and so we use this now. | ||
For longer storage up to 5 days in the fridge (+ 4°C) TB medium can be used. It has a smal background fluorescence in WatsOn. | For longer storage up to 5 days in the fridge (+ 4°C) TB medium can be used. It has a smal background fluorescence in WatsOn. |
Revision as of 18:41, 15 October 2014
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