Team:Aachen/Project/2D Biosensor
From 2014.igem.org
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== Medium == | == Medium == | ||
- | Prior to using our own device for detection of fluorescence emitted by the sensor chips we used equipment readily available in the lab. A Molecular Imager® Gel Doc<sup>TM</sup> XR+ from BIO-RAD was available which used UV and white light illuminators. Only two different filters were availble for the excitation ligth wavelength, which resulted in | + | Prior to using our own device for detection of fluorescence emitted by the sensor chips we used equipment readily available in the lab. A Molecular Imager® Gel Doc<sup>TM</sup> XR+ from BIO-RAD was available which used UV and white light illuminators. Only two different filters were availble for the excitation ligth wavelength, which resulted in a very limitted range of light wavelengths available for excitation of fluorescent molecules. For example, it was possible to detect the expression of iLOV in our sensor chips, but in contrast detection of GFP was not possible. Thus the Gel Doc<sup>TM</sup> XR+ was not ideal for our project. |
(iLOV_GFP_HM_1,5h.png) | (iLOV_GFP_HM_1,5h.png) | ||
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- | Concerning the medium used for our sensor chips, LB medium showed a high | + | Concerning the medium used for our sensor chips, LB medium showed a high background fluorescence when exposed to UV light. Surprinsingly the background fluorescence resulting from the LB medium was to high to detect a signal emitted by our sensor cells, thus we used minimal media (NA, M9, Hartman) in order to minimize the background fluorescence. The appliction of minimal media was sufficient to minimize the background fluorescence, but this approach came with the drawback of minimal to zero growth of our sensor cells. |
(Chip_medium_geldoc.png) | (Chip_medium_geldoc.png) | ||
Revision as of 18:38, 15 October 2014
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