Team:Aachen/Project/2D Biosensor
From 2014.igem.org
(→Principle of Operation) |
(→Principle of Operation) |
||
Line 65: | Line 65: | ||
{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Application of WatsOn for investigation of solid surfaces.|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Application of WatsOn for investigation of solid surfaces.|width=1000px}} | ||
- | The application of ''Cellock Holmes'' for detection of ''P. aeruginosa'' cells is simple: fist, a sampling chip is placed on a hard surface that is potentially contaminated with the pathogen. Second, the chip is put onto one of our sensor chips | + | The application of ''Cellock Holmes'' for detection of ''P. aeruginosa'' cells is simple: fist, a sampling chip is placed on a hard surface that is potentially contaminated with the pathogen. Second, the sampling chip is removed from the surface and put onto one of our sensor chips. Theorectically the sensor chip could be directly used for sampling, but this was avoided in our project for safety reasons. Third, the two layered chip-stack is put into a petri dish, which is inserted into our measurement device [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] for evalutation. |
{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=Mode of action inside WatsOn.|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=Mode of action inside WatsOn.|width=1000px}} | ||
- | Inside ''WatsOn'' the chips are incubated at 37 °C and populations of microorganisms on the sampling | + | Inside ''WatsOn'' the chips are incubated at 37 °C and populations of microorganisms on the sampling chips start to multiply. ''P. aeruginosa'' secrets an increasing number of 3-oxo-C{{sub|12}} homoserine lactones (HSLs) when multiplying. |
The chips can be illuminated with blue light at any time, while ''WatsOn'' takes a picture of the chip. The software ''Measurarty'' then analyzes any fluorescent signal. Depending on the intensity of the signal and the size of the spot, ''Cellock Holmes'' can '''calculate concentration and distribution of ''P. aeruginosa'' ''' on the sampled surface. | The chips can be illuminated with blue light at any time, while ''WatsOn'' takes a picture of the chip. The software ''Measurarty'' then analyzes any fluorescent signal. Depending on the intensity of the signal and the size of the spot, ''Cellock Holmes'' can '''calculate concentration and distribution of ''P. aeruginosa'' ''' on the sampled surface. | ||
Revision as of 15:00, 15 October 2014
|
|
|