Team:Aachen/Project/2D Biosensor
From 2014.igem.org
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{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Flow chart of chip measurement using WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Flow chart of chip measurement using WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | ||
- | The application of ''Cellock Holmes'' for detection of ''P. aeruginosa'' cells is simple: fist, a sampling chip is placed on a hard surface that is potentially contaminated with the pathogen. Subsequently, the chip is put onto on of our sensor chips. The two layered chip-stack is then put into a patri dish, which is inserted | + | The application of ''Cellock Holmes'' for detection of ''P. aeruginosa'' cells is simple: fist, a sampling chip is placed on a hard surface that is potentially contaminated with the pathogen. Subsequently, the chip is put onto on of our sensor chips. The two layered chip-stack is then put into a patri dish, which is inserted into our measurement device [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] for evalutation. |
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{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=What happens inside WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=What happens inside WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | ||
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+ | introduced where it is incubated at 37 °C. | ||
After a short time, the chip is illuminated with blue light. ''WatsOn'' takes a picture of the chip and the software ''Measurarty'' analyzes any fluorescent signal. Depending on the intensity of the signal and the size of the spot, ''Cellock Holmes'' can '''calculate concentration and distribution of ''P. aeruginosa'' ''' on the sampled surface. | After a short time, the chip is illuminated with blue light. ''WatsOn'' takes a picture of the chip and the software ''Measurarty'' analyzes any fluorescent signal. Depending on the intensity of the signal and the size of the spot, ''Cellock Holmes'' can '''calculate concentration and distribution of ''P. aeruginosa'' ''' on the sampled surface. | ||
Revision as of 13:54, 15 October 2014
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