Team:Aachen/Project/2D Biosensor
From 2014.igem.org
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- | ''Cellock Holmes'' is devised based upon a SynBio approach comprised of a '''two-dimensional biosensor and a measurement device'''. The two-dimensional biosensor is designed to recognize quorum sensing molecules secreted by the pathogen cells and to generate a distinct fluorescence signal, while the measurement device is designed to recognize and analyse the produced signal. To overcome the limitation of our fluorescence based [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh construct] to bacteria that secrete autoinducers, we developed an | + | ''Cellock Holmes'' is devised based upon a SynBio approach comprised of a '''two-dimensional biosensor and a measurement device'''. The two-dimensional biosensor is designed to recognize quorum sensing molecules secreted by the pathogen cells and to generate a distinct fluorescence signal, while the measurement device is designed to recognize and analyse the produced signal. To overcome the limitation of our fluorescence based [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter REACh construct] to bacteria that secrete autoinducers, we developed an alternative detection method ([https://2014.igem.org/Team:Aachen/Project/Gal3 Galectin-3 construct]), which is based on tagging cells with a fluorescent reporter. |
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[graph quorum senising] | [graph quorum senising] |
Revision as of 11:21, 15 October 2014
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