Team:Aachen/Project/2D Biosensor
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Our '''sensor cells are immobilized in agar chips'''. To make the chips, we mix the sensing cells, also known as ''Cellocks'', with liquid LB agar. In the course of our project, we designed a casting mold specifically for the production of our agar chips. When the agar has cooled down, the chips are cut out of the mold and ready to use . If you want to know exactly how our chips are manufactures, you can read up on more details in our [https://2014.igem.org/Team:Aachen/Notebook/Protocols/detection Protocols]. | Our '''sensor cells are immobilized in agar chips'''. To make the chips, we mix the sensing cells, also known as ''Cellocks'', with liquid LB agar. In the course of our project, we designed a casting mold specifically for the production of our agar chips. When the agar has cooled down, the chips are cut out of the mold and ready to use . If you want to know exactly how our chips are manufactures, you can read up on more details in our [https://2014.igem.org/Team:Aachen/Notebook/Protocols/detection Protocols]. | ||
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<center> | <center> | ||
{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Flow chart of chip measurement using WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part1 ipo.png|title=Flow chart of chip measurement using WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | ||
+ | To detect ''P. aeruginosa'' cells, the chip is placed on a hard surface that is potentially contaminated with the pathogen. Subsequently, the chip is introduced into our measurement device [https://2014.igem.org/Team:Aachen/Project/Measurement_Device ''WatsOn''] where it is incubated at 37 °C. | ||
{{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=What happens inside WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | {{Team:Aachen/Figure|Aachen 14-10-14 Flowsheet OD-device part2 ipo.png|title=What happens inside WatsOn.|subtitle=NOT FINISHED!|width=1000px}} | ||
+ | After a short time, the chip is illuminated with blue light. ''WatsOn'' takes a picture of the chip and the software ''Measurarty'' analyzes any fluorescent signal. Depending on the intensity of the signal and the size of the spot, ''Cellock Holmes'' can '''calculate concentration and distribution of ''P. aeruginosa'' ''' on the sampled surface. | ||
</center> | </center> | ||
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Revision as of 10:39, 15 October 2014
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