iGEM Team Aachen BioBricks

K1319000

RFC [25] Version of E0020

This part is an RFC [25]-compatible version of BBa_E0030. The start and stop codons have been removed to make it RFC [25]-compatible and the part is flanked by the RFC [25] prefix- and suffix-sequences.

K1319001

RFC [25] -compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Two mutations were introduced that eliminated fluorescence:

• L90I
• Y145W

K1319002

RFC [25] -compatible dark quencher based on K1319000 (E0030)

This part is an RFC [25] dark quencher that is based upon K1319000 (the RFC [25] version of E0030/EYFP). Three mutations were introduced that eliminated fluorescence:

• L90I
• Y145W
• H148R

K1319003

human galectin-3, codon optimized for E. coli

The trunkated galectin-3 is a 26 kDa protein that binds certain LPS patterns.

K1319004

TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli

This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.

K1319020

Translational unit of mRFP-galectin3-his

This part is a translational unit of a mRFP-galectin-3-his (B0032.E1010.K1319003.K1319016.B0015).

K1319042

IPTG inducible iLOV

This part can be used for IPTG-induced expression of K660004 (iLOV).

1. Part Name
2. Part type
3. Creator
4. Sequence
5. Short Description (60 characters on what the DNA does)
6. Long Description (Longer description of what the DNA does)
7. Design considerations

<groupparts>iGEM14 Aachen</groupparts>