Team:Aachen/OD/F device
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The measuring principle for both optical density (OD) and fluorescence measurement is depicted below. | The measuring principle for both optical density (OD) and fluorescence measurement is depicted below. | ||
- | For OD measurement we shine through the sample with an LED and a fixed width. A filter blocks any other light but 600 nm. This way, the sensor mainly senses the 600 nm light which is needed for | + | For OD measurement we shine through the sample with an LED and a fixed width. A filter blocks any other light but 600 nm. This way, the sensor mainly senses the 600 nm light which is needed for OD<sub>600</sub> measurement. |
For fluorescence measurement a similar approach is chosen. The filter again is used to block the exciting light from being sensed. That way only the emitted light from the fluorescence protein is measured. | For fluorescence measurement a similar approach is chosen. The filter again is used to block the exciting light from being sensed. That way only the emitted light from the fluorescence protein is measured. |
Revision as of 18:30, 16 October 2014
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