Team:Aachen/Notebook/Wetlab/June
From 2014.igem.org
(Difference between revisions)
AZimmermann (Talk | contribs) (Created page with "__NOTOC__ {{Team:Aachen/Header}} = June = == 3rd == * transformation of 34 BioBricks == 4th == * preperation of consumables: ** fresh 50 % glycerol ** new LB plates with...") |
(→June) |
||
Line 29: | Line 29: | ||
'''PCR of E0030 to K1319000''' | '''PCR of E0030 to K1319000''' | ||
* Q5 and Phusion polymerase are used | * Q5 and Phusion polymerase are used | ||
+ | <center> | ||
{| class="wikitable" | {| class="wikitable" | ||
! parameter !! duration !! temp [°C] | ! parameter !! duration !! temp [°C] | ||
Line 42: | Line 43: | ||
| elongate||05:00||72 | | elongate||05:00||72 | ||
|} | |} | ||
+ | </center> | ||
→ 30 cycles | → 30 cycles | ||
== 17th == | == 17th == | ||
* colony PCR on following constructs: | * colony PCR on following constructs: | ||
+ | <center> | ||
{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
Line 62: | Line 65: | ||
| 11 || negativ control || - | | 11 || negativ control || - | ||
|} | |} | ||
+ | </center> | ||
→ anneling: 50°C | → anneling: 50°C | ||
Line 69: | Line 73: | ||
* colony PCR of K325108, K325218, C0062 | * colony PCR of K325108, K325218, C0062 | ||
+ | <center> | ||
{| class="wikitable" | {| class="wikitable" | ||
! parameter !! duration !! temp [°C] | ! parameter !! duration !! temp [°C] | ||
Line 82: | Line 87: | ||
| elongate||05:00||72 | | elongate||05:00||72 | ||
|} | |} | ||
+ | </center> | ||
→ 30 cycles | → 30 cycles | ||
* PAGE: 30 min on 1.2 % agarose gel at 110 V | * PAGE: 30 min on 1.2 % agarose gel at 110 V | ||
Line 114: | Line 120: | ||
== 27th == | == 27th == | ||
* plasmid preps | * plasmid preps | ||
+ | <center> | ||
{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
Line 134: | Line 141: | ||
| J23101.E0240 #6 || 229.5 | | J23101.E0240 #6 || 229.5 | ||
|} | |} | ||
+ | </center> | ||
* two cryos stocks of each were prepared | * two cryos stocks of each were prepared | ||
Revision as of 14:57, 8 October 2014
|