Media

LB medium

Hartmans minimal medium (HM)

Agar Chips

Transformation

Heat Shock

1. thaw cells on ice
2. add 1 µl of plasmid DNA
3. incubate on ice for 30'
4. heat shock at 42 °C for 60
5. incubate on ice for 5'
6. add 200 µl of SOC media
7. incubate at 37 °C for 2 h
8. plate 20  and 200 µl on plates with the appropiate antibiotic

Electroporation

PCR

Colony PCR

QuikChange

Clonings

Restriction Digest

Ligation

Gibson Assembly