Team:Aachen/Notebook/Protocols
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(→Agar Chips) |
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# Centrifuge all 50 mL by 3000 g for 10 min at RT (21 °C). | # Centrifuge all 50 mL by 3000 g for 10 min at RT (21 °C). | ||
# Discard the supernatant | # Discard the supernatant | ||
- | # Re-suspend the pellet in 1 mL tempered(~21 °C) LB-medium . | + | # Re-suspend the pellet in 1 mL tempered (~21 °C) LB-medium . |
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# Mix the cooled medium with the cells by inverting gently. | # Mix the cooled medium with the cells by inverting gently. | ||
# Pour it in the chip form, avoiding bubble formation (!). | # Pour it in the chip form, avoiding bubble formation (!). | ||
- | # Wait for | + | # Wait for approximately 20 min until the agar has solidified. |
# Cut out the chips with a scalpel. | # Cut out the chips with a scalpel. | ||
# Put two chips into a labeled petri dish and store additional 4 chips in labeled petri dishs in the refrigerator. | # Put two chips into a labeled petri dish and store additional 4 chips in labeled petri dishs in the refrigerator. |
Revision as of 16:10, 6 October 2014
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