Team:Aachen/Notebook/Engineering/WatsOn
From 2014.igem.org
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{{Team:Aachen/FigureFloat|Aachen_Device_11.jpg|title=WatsOn|subtitle= |width=200px}} | {{Team:Aachen/FigureFloat|Aachen_Device_11.jpg|title=WatsOn|subtitle= |width=200px}} | ||
- | Our hardware consists of the casing and the electronical components. The casing which can be seen on the left was | + | Our hardware consists of the casing and the electronical components. The casing which can be seen on the left was built from laser cut acrylic glass. A detailed description of the assembly is described in the section [https://2014.igem.org/Team:Aachen/Notebook/Engineering/WatsOn#watsondiy ''Build your own WatsOn'']. |
- | ''Download the laser cutting plan here: [https://2014.igem.org/File:Aachen_WatsOn_laser_cut.svg.zip Download] (for acrylic glass with a height of 6 mm) | + | |
+ | ''Download the laser cutting plan here: [https://2014.igem.org/File:Aachen_WatsOn_laser_cut.svg.zip Download] (for acrylic glass with a height of 6 mm) | ||
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The connection between the different electronical elements is visualizes below. | The connection between the different electronical elements is visualizes below. | ||
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{{Team:Aachen/Figure|Aachen_Device_Hardware_Graphics.png|title=Eletronical components||width=750px}} | {{Team:Aachen/Figure|Aachen_Device_Hardware_Graphics.png|title=Eletronical components||width=750px}} | ||
- | * '''Raspberry Pi''' : The Raspberry Pi is a small single-board computer which runs a Linux operating system from an inserted SD | + | * '''Raspberry Pi''' : The Raspberry Pi is a small single-board computer which runs a Linux operating system from an inserted SD card. The steps which are required to set up a fully working system are described in the [https://2014.igem.org/Team:Aachen/Notebook/Engineering/WatsOn#pisetup DIY section] of this page. The main purpose of the Raspberry Pi is to run the software described above, to control the attached camera and to show the GUI on the display. The big advantage of this board is that it is very powerful, cheap and therefore perfectly fit for our needs. |
* '''Raspberry Pi camera''': The camera is directly connected to the Raspberry Pi board and takes the images of the chips. | * '''Raspberry Pi camera''': The camera is directly connected to the Raspberry Pi board and takes the images of the chips. | ||
- | * '''Arduino''': The arduino board is also a single-board computer with less computing power than the Raspberry Pi but with a greater focus on controlling electronical components. Therefore it is used to control the LEDs and the Peltier heater. | + | * '''Arduino''': The arduino board is also a single-board computer with less computing power than the Raspberry Pi but with a greater focus on controlling electronical components. Therefore, it is used to control the LEDs and the Peltier heater. |
- | * '''Relay''': The 2-channel relay works like two light switches which | + | * '''Relay''': The 2-channel relay works like two light switches which are either turned on or off. They control the 450 nm and 480 nm LEDs. The channels are connected and turned on and off by the arduino board. |
- | * '''Peltier element''': A Peltier component transforms an applied power into a temperature gradient which leads to a hot surface on one side of the element and a cooler one on the other. The Peltier element connected to the | + | * '''Peltier element''': A Peltier component transforms an applied power into a temperature gradient which leads to a hot surface on one side of the element and a cooler one on the other side. The Peltier element connected to the aluminum block heats up the interior of the device to incubate the sensing cells at 37 °C. |
* '''USB WiFi stick''': The USB WiFi stick connects the Raspberry Pi to a local network. | * '''USB WiFi stick''': The USB WiFi stick connects the Raspberry Pi to a local network. | ||
- | * '''Display''': | + | * '''Display''': An 8-digit display is connected to the arduino board and shows the current interior temperature |
{{Team:Aachen/FigureFloatRight|Aachen_Filter_010.png|title=010|subtitle=|width=70px}} | {{Team:Aachen/FigureFloatRight|Aachen_Filter_010.png|title=010|subtitle=|width=70px}} | ||
{{Team:Aachen/FigureFloatRight|Aachen_Filter_505.png|title=505|subtitle=|width=70px}} | {{Team:Aachen/FigureFloatRight|Aachen_Filter_505.png|title=505|subtitle=|width=70px}} | ||
- | * '''Filter slides''': To block the | + | * '''Filter slides''': To block the undesired wavelenghts emitted from the LEDs a filter slide is placed in front of the camera. This step is taken to get a clear fluorescence signal from the chips. The characteristic of the filter slide is selected depending on the frequency of the LEDs which are either 450 nm or 480 nm ones. We used '505 Sally Green' for the 450 nm and '010 Medium Yellow' for the 480 nm LEDs. The filters are shown on the right. |
{{Team:Aachen/BlockSeparator}} | {{Team:Aachen/BlockSeparator}} |
Revision as of 08:02, 17 October 2014
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