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| In order to detect the pathogens fast, specifically and inexpensively we are building sensor cells to detect these pathogens. These sensor cells can identify pathogens in very low concentration by responsing to specific extracellular molecules either secreted by or displayed on the pathogens. These molecules trigger a fast fluorescence response by our immobilized sensor cells which will be measured by our device.
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More information will be available soon!
May
1st to 11th
Thu, 8th
12th to 18th
23rd
- we transformed some BioBricks and did a colony PCR
26th to 31st
June
25th
- preculture for competent NEB10β cells
- submit samples for sequencing
- make masterplates of transformed BioBricks
- centrifuge and freeze overnight expression culture of J23101.E0240 and K516132
26th
- made competent NEB10β cells - several things went wrong.. we'll see (remember: pre-cool centrifuge, always check if it's indeed spinning, frequently check OD of the culture)
- colony PCR on the transformed clones looked awful. There were too many cells in the 10 µl reaction volume. Some seals weren't fully closed. Basically only primers and smear except for the positive control which contained a plasmid template instead of cells
- 2x 500 ml of LB and three sterile flasks have been made
July
16th
- plasmid preps
- transformation of different reporter strains
- ...
sample table:
BioBrick | Colonies after Transformation | Colony PCR result
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E0240 | 5 | positive
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E0040 | 6 | negative
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22nd
- Philipp and Michael made 100x stocks for Hartmanns media
- Michael and Vera made about 25 HM+C-plates without Glucose
- K731520 iLOV was plated on a HM+C plate
23rd
- Michael made 25 new HM+C plates with 1% agar and 4 g/L glucose
- Michael also added 170 µl of the Glucose stock (500 g/L) to the Glucose-free HM+C-plates
August
...
September
...
October
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