Team:Aachen/Labbook
From 2014.igem.org
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| Thiamine hydrochloride || 0.3 || 300 mg/L | | Thiamine hydrochloride || 0.3 || 300 mg/L | ||
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- | | | + | | MgSO{{sub|4}} || 0.208 || 2 mmol/L |
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- | | | + | | CaCl{{sub|2}} || 0.011 || 0.1 mmol/L |
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The powders were dissolved in a 10 % w/v Casamino acid stock solution and sterile-filtered (.22 µm PES). 20 ml of the supplement stock can be added to 1 L of 1x HM media. To make agar chips, we can add 1 ml to the hot agar mixture, together with the three 500 µl 100x stocks for the HM. | The powders were dissolved in a 10 % w/v Casamino acid stock solution and sterile-filtered (.22 µm PES). 20 ml of the supplement stock can be added to 1 L of 1x HM media. To make agar chips, we can add 1 ml to the hot agar mixture, together with the three 500 µl 100x stocks for the HM. |
Revision as of 10:59, 24 July 2014
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