Interlab Study

Summary

Results

Discussion

Materials and Methods

Constructs and strains

All constructs used were transformed into NEB 10 Beta cells. The constructs I20260 as well as B0015 were taken directly from the iGEM 2014 distribution plates and transformed directly. The constructs J23101.E0240 as well as J23115.E0240 were made using the 3A Assembly.

Inoculation and Cultivation

Sampling

Measurement of OD using a Spektrophotometer

Measurement of fluorescence using a Platereader

Measurement of OD and fluorescence using the combined OD/F device of the iGEM Team Aachen 2014