Team:Aachen/Interlab Study
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We introduced the three constructs in to ''E. coli'' cells and measured fluorescence as well as optical density of the liquid cultures over a period of 18 hours, using a spectrophotometer and a plate reader, respectively. The obtained results confirmed our hypothesis that the fluorescence of the BioBrick in the high copy plasmid pSB1C3, J23101.E0240, would exhibit a stronger signal than the constructs I20260, which is on the low to mid copy plasmid pSB3K3, and J23115.E0240, which has a weaker promotor than J23101.E0240. During the experiment, we could observe a typical growth curve for ''E.coli'' including lag, exponential, stationary and death phase. We could show that the fluorescence we measured is rather a function of each cell than the whole culture, since all cultures had comparable optical densities. | We introduced the three constructs in to ''E. coli'' cells and measured fluorescence as well as optical density of the liquid cultures over a period of 18 hours, using a spectrophotometer and a plate reader, respectively. The obtained results confirmed our hypothesis that the fluorescence of the BioBrick in the high copy plasmid pSB1C3, J23101.E0240, would exhibit a stronger signal than the constructs I20260, which is on the low to mid copy plasmid pSB3K3, and J23115.E0240, which has a weaker promotor than J23101.E0240. During the experiment, we could observe a typical growth curve for ''E.coli'' including lag, exponential, stationary and death phase. We could show that the fluorescence we measured is rather a function of each cell than the whole culture, since all cultures had comparable optical densities. | ||
+ | <center> | ||
{{Team:Aachen/Figure|Interlabstudy_overview_wiki.png|title=Fig. 1 Interlab Study Results|subtitle=Our measurements of fluorescence and optical density of the three genetic devices and a negative control.|width=1000px}} | {{Team:Aachen/Figure|Interlabstudy_overview_wiki.png|title=Fig. 1 Interlab Study Results|subtitle=Our measurements of fluorescence and optical density of the three genetic devices and a negative control.|width=1000px}} | ||
{{Team:Aachen/BlockSeparator}} | {{Team:Aachen/BlockSeparator}} | ||
+ | </center> | ||
==Experimental Design== | ==Experimental Design== |
Revision as of 16:07, 8 October 2014
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