Team:Aachen/Interlab Study
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AZimmermann (Talk | contribs) (→Inoculation and Cultivation) |
AZimmermann (Talk | contribs) (→Inoculation and Cultivation) |
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The cultivation of our cultures was performed in 50 ml [https://2014.igem.org/Team:Aachen/Notebook/Protocols#LB_medium LB Media] in 500ml shake flasks at 37 degrees Celsius and 300rpm shaking frequency. | The cultivation of our cultures was performed in 50 ml [https://2014.igem.org/Team:Aachen/Notebook/Protocols#LB_medium LB Media] in 500ml shake flasks at 37 degrees Celsius and 300rpm shaking frequency. | ||
- | The precultures were inoculated from the same cryo stock every time. They were cultivated for 16 hours and then sampled for OD measurement with a Spektrophotometer. Then 2ml of each preculture were centrifuged (5 minutes, 6000g) and then washed twice with PBS buffer. Afterwards all cultures were inoculated to have the same OD. | + | The precultures were inoculated from the same cryo stock every time. They were cultivated for 16 hours and then sampled for OD measurement with a Spektrophotometer. Then 2ml of each preculture were centrifuged (5 minutes, 6000g) and then washed twice with PBS buffer. Afterwards all cultures were inoculated to have the same starting OD. |
===Sampling=== | ===Sampling=== |
Revision as of 11:52, 25 September 2014
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