Team:Aachen/Collaborations/Freiburg
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= AcCELLoMatrix with [[Team:Freiburg|Team Freiburg]]= | = AcCELLoMatrix with [[Team:Freiburg|Team Freiburg]]= | ||
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- | + | At the iGEM meetup in Munich in May , members of both of our teams realized that our projects share a common objective: Analyzing 2-dimensional, visual signals. For the following months, we stayed in contact and developed a concept to encode information in 384-well plates in the form of data matrix codes. | |
+ | At the beginning of September, Michael spontaneously took a train to Freiburg and we met face-to-face for several hours to exchange details on our cooperation and general iGEM experiences. | ||
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+ | <center> | ||
{{Team:Aachen/Figure|Aachen_FR-collaboration_group_photo.jpg|title=Spontaneous group photo|subtitle=Freíburg iGEMers and Michael (right) meeting to discuss our cooperation|width=350px}} | {{Team:Aachen/Figure|Aachen_FR-collaboration_group_photo.jpg|title=Spontaneous group photo|subtitle=Freíburg iGEMers and Michael (right) meeting to discuss our cooperation|width=350px}} | ||
- | + | </center> | |
== Data Matrix Masks for illumination == | == Data Matrix Masks for illumination == | ||
- | We decided to use masks to locally induce the cells in 384-well plates in a | + | |
+ | We decided to use masks to locally induce the cells in 384-well plates in a data matrix pattern. To enable us to quickly design masks for different data to be encoded, we wrote a software to easily generate SVG-files for a laser cutter. | ||
+ | |||
+ | <center> | ||
{{Team:Aachen/Figure|Aachen_DataMatrixMaskDesigner.png|title=Making a datamatrix mask|subtitle=Upon entering a string, the software predicts if the datamatrix will fit into a 384-well plate.|width=350px}} | {{Team:Aachen/Figure|Aachen_DataMatrixMaskDesigner.png|title=Making a datamatrix mask|subtitle=Upon entering a string, the software predicts if the datamatrix will fit into a 384-well plate.|width=350px}} | ||
+ | </center> | ||
- | After a string | + | After a string has been entered in the designer, an SVG-file can be saved and used for laser cutting the mask: |
+ | <center> | ||
{{Team:Aachen/Figure|Aachen_MammoMatrixMasks2.png|title=Cut Lines|subtitle=This AcCELLoMatrix mask can be lasercut and placed below a 384-well plate.|width=350px}} | {{Team:Aachen/Figure|Aachen_MammoMatrixMasks2.png|title=Cut Lines|subtitle=This AcCELLoMatrix mask can be lasercut and placed below a 384-well plate.|width=350px}} | ||
+ | </center> | ||
[https://static.igem.org/mediawiki/2014/a/a8/Aachen_DatamatrixMaskDesigner.zip Get the DatamatrixMaskDesigner] to make you own MammoMatrixes! (requires Windows 7/8) | [https://static.igem.org/mediawiki/2014/a/a8/Aachen_DatamatrixMaskDesigner.zip Get the DatamatrixMaskDesigner] to make you own MammoMatrixes! (requires Windows 7/8) | ||
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You can also [https://static.igem.org/mediawiki/2014/c/ce/Aachen_DatamatrixMaskDesigner_Source.zip download the full source code] and compile or modify it with Visual Studio (Express) 2013. | You can also [https://static.igem.org/mediawiki/2014/c/ce/Aachen_DatamatrixMaskDesigner_Source.zip download the full source code] and compile or modify it with Visual Studio (Express) 2013. | ||
- | For the first experiments we cut two different | + | For the first experiments, we cut two different data matrix patterns from polypropylen foil. The masks can be used to cover the plates from the top or from the bottom. |
- | {{Team:Aachen/Figure|Aachen_MammoMatrixMasks1.jpg|title=AcCELLoMatrix masks|subtitle=These masks can be placed on top | + | {{Team:Aachen/Figure|Aachen_MammoMatrixMasks1.jpg|title=AcCELLoMatrix masks|subtitle=These masks can be placed on top or beneath 384-well plates to facilitate localized illumination of wells.|width=350px}} |
{{Team:Aachen/Footer}} | {{Team:Aachen/Footer}} |
Revision as of 19:03, 11 October 2014
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