Team:Evry/Notebook/Sensing/PCBs/week8

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Week 8

Interlab Study

08.18.2014

Received primers:

08.19.2014

We want to amplify these two parts: BBa_E1010 and BBa_B0015. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.
A PCR was perform for amplification with the Q5 polymerase and dedicated primers for Golden Gate: 45 and 46 (BBa_E1010) ; 47 and 48 (BBa_B0015).
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Table 1: PCR mix Preparation for Q5 amplification.
The used program was described on table 1. There was a difference for BBa_B0015, for the 72°C phase time= 15 seconds and no 30.
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Table 1: IGEM Q5 PCR program thermocycling conditions

08.20.2014

08.21.2014

08.22.2014

08.23.2014

08.24.2014

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