Team:LIKA-CESAR-Brasil/TheRobot

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LIKA | CESAR

NOTEBOOK

Materials:

1.Control Unit

  • 1.Arduino UNO

2.Power Supply

  • 1.Computer Power Supply Unit (ATX)
  • 2.Custom designed plate | 2.1. 5V and 12V conectors

3.Electric Syringe

  • 1.Stteper motor
  • 2.Endless worm
  • 3.Custom designed parts
  • 4.Syringe
  • 5.IV extension tubes
  • 6.3-Way Stopcock

4.Robotic Arm

  • 1.Servo motors
  • 2.Custom designed parts

5.Centrifuge

  • 1.Brushless motor
  • 2.Esc for motor
  • 3.Centrifuge arm (custom designed)
  • 4.Motor support (custom designed)
  • 5.PCR tube support (custom designed)
  • 2.Esc for motor

1.Done manually:

  • 1.Collect a 2 mL blood sample from patient in EDTA tube with 2 mL of RNA later;
  • 2.Take a 500 μL fraction from the collected blood sample and transfer to 1.5mL PCR tubes;

2.Robot:

  • 1.Adds 1mL of lysis solution (custom designed) in the 500 μL of blood, mix and wait 10 minutes;
  • 2.After 10 minutes, heat the PCR tube at 56°C for 10 minutes;
  • 3.Centrifuge at 8.000 rpm for 5 minutes;
  • 4.Heat the PCR tube at 98ºC for 1min;
  • 1.Take 5 μL of the supernatant part of the liquid inside the PCR tube to the biosensor.
Marca LIKA Marca CESAR