Team:Caltech/Project/Methods and Methods

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Materials and Methods
Overall Project Summary

Project Details

Materials and Methods

The Experiments

Results

Data Analysis

Conclusions

References

PCR
  • blah blah
  • blah blah

Wednesday, 6/18/14
  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject

Thursday, 6/19/14
  • Formulated plasmid design (pAA001) for the response regulation subproject
  • PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
  • With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14
  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
  • Colonies were found and picked after transformation and incubation.

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