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Team:Bielefeld-CeBiTec/Results/Outlook
From 2014.igem.org
Module III - Isobutanol production
Isobutanol pathway
Alcohol dehydrogenase
As described in the section about the alcohol dehydrogenase we could show the successful overexpression of the protein AdhA from Lactococcus lactis in E. coli.
Further analysis could be an anaerobic cultivation for a complementation analysis of the strain ΔadhE748::kan. Trinh et al., 2011 showed that E. coli cannot grow after the loss of its alcohol dehydrogenase.
References
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Trinh CT, Li J, Blanch HW, Clark DS., 2011. Redesigning Escherichia coli Metabolism for Anaerobic Production of Isobutanol. In: Appl Environ Microbiol., 77(14): 4894-904
