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Team:Evry/Notebook/Sensing/PCBs/week8
From 2014.igem.org
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| + | <center>Table 1: Received Primer Sensor construction hbpR/hbpC </center> | ||
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| - | <center>Table | + | <center>Table 3: IGEM Q5 PCR program thermocycling conditions </center> |
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Revision as of 15:39, 19 August 2014
Week 8
Interlab Study
08.18.2014
08.19.2014
We want to amplify these two parts: BBa_E1010 and BBa_B0015. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.A PCR was perform for amplification with the Q5 polymerase and dedicated primers for Golden Gate: 45 and 46 (BBa_E1010) ; 47 and 48 (BBa_B0015).
