Team:Caltech/Project/Methods and Methods

From 2014.igem.org

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<h4>Wednesday, 6/18/14</h4>
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<h4>Gel electrophoresis</h4>
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<ul><li>Continue to discuss project and clean up lab space</li>
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<ul><li>whreeeee</li>
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     <li>Designed PCR primers for combinatorial promoters subproject</li>
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     <li>blah blah</li>
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<h4>Thursday, 6/19/14</h4>
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<h4>Colony PCR</h4>
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<ul><li>Formulated plasmid design (pAA001) for the response regulation subproject</li>
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<ul><li>blah blah</li>
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     <li>PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
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     <li>blah blah</li>
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     <li>Gel electrophoresis for confirmation of PCR products' length</li>
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     <li>blah blah</li>
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    <li>blah blah</li>
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<ul><li>With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</li><ul>
 
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<h4>Friday, 6/20/14</h4>
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<h4>Gibson assembly</h4>
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<ul><li>Purification of PCR products created yesterday</li>
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<ul><li>whreee</li>
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     <li>Gibson assembly of purified products</li>
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     <li>blah blah</li>
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     <li>Transformation of Gibson-assembled plasmids into JM109 E. coli</li>
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     <li>blah blah</li>
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     <li>Meeting with Prof. Murray to discuss give an update on the project</li>
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     <li>blah blah</li>
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    <li>blah blah</li>
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<ul><li>Colonies were found and picked after transformation and incubation.</li></ul>
 
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Revision as of 18:53, 14 July 2014



Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions
Materials and Methods
Overall Project Summary

Project Details

Materials and Methods

The Experiments

Results

Data Analysis

Conclusions

References

PCR
  • blah blah
  • blah blah

Gel electrophoresis
  • whreeeee
  • blah blah

Colony PCR
  • blah blah
  • blah blah
  • blah blah
  • blah blah

Gibson assembly
  • whreee
  • blah blah
  • blah blah
  • blah blah
  • blah blah

Retrieved from "http://2014.igem.org/Team:Caltech/Project/Methods_and_Methods"