Team:Caltech/Project/Methods and Methods

From 2014.igem.org

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<h4>PCR</h4>
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<ul><li>blah blah</li>
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    <li>blah blah</li>
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<h4>Wednesday, 6/18/14</h4>
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<ul><li>Continue to discuss project and clean up lab space</li>
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    <li>Designed PCR primers for combinatorial promoters subproject</li>
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<h4>Thursday, 6/19/14</h4>
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<ul><li>Formulated plasmid design (pAA001) for the response regulation subproject</li>
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    <li>PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly</li>
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    <li>Gel electrophoresis for confirmation of PCR products' length</li>
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<ul><li>With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis</li><ul>
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<h4>Friday, 6/20/14</h4>
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<ul><li>Purification of PCR products created yesterday</li>
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    <li>Gibson assembly of purified products</li>
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    <li>Transformation of Gibson-assembled plasmids into JM109 E. coli</li>
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    <li>Meeting with Prof. Murray to discuss give an update on the project</li>
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<ul><li>Colonies were found and picked after transformation and incubation.</li></ul>
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Revision as of 18:40, 14 July 2014



Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions
Materials and Methods
Overall Project Summary

Project Details

Materials and Methods

The Experiments

Results

Data Analysis

Conclusions

References

PCR
  • blah blah
  • blah blah

Wednesday, 6/18/14
  • Continue to discuss project and clean up lab space
  • Designed PCR primers for combinatorial promoters subproject

Thursday, 6/19/14
  • Formulated plasmid design (pAA001) for the response regulation subproject
  • PCR run on plasmid backbone and sfGFP to create constructs with the proper overhangs for Gibson assembly
  • Gel electrophoresis for confirmation of PCR products' length
  • With the exception of the lacI gene, PCR products were confirmed via gel electrophoresis

Friday, 6/20/14
  • Purification of PCR products created yesterday
  • Gibson assembly of purified products
  • Transformation of Gibson-assembled plasmids into JM109 E. coli
  • Meeting with Prof. Murray to discuss give an update on the project
  • Colonies were found and picked after transformation and incubation.

Retrieved from "http://2014.igem.org/Team:Caltech/Project/Methods_and_Methods"