Team:GeorgiaTech/Project/Primers

From 2014.igem.org

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<p>The RBS primer is a standard primer that can be used for site directed mutagenesis in all linearized backbones available from the iGEM database.</p></br>
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<p>The RBS primer is a standard primer that can be used for site directed mutagenesis in all linearized backbones available from the iGEM database.</p><p></p>
<h2>Background</h2>
<h2>Background</h2>

Revision as of 17:43, 11 July 2014

Biobrick Primers for Site-Directed Insertion Mutagenesis

Ribosomal Binding Site Insertion

Purpose

The RBS primer is a standard primer that can be used for site directed mutagenesis in all linearized backbones available from the iGEM database.

Background

Most of the BioBrick parts requires a Ribosomal Binding Site to express the protein. This has proven problematic as the RBS is very small and hard to insert using 3A Assembly. We believe the creation of an oligo primer with this RBS may be easier to place the RBS in front of the protein that is needed to be expressed. An easy oligo primer that could be used in PCR extension and then placed into a vector backbone would save time and frustration as to whether the part had been inserted.

Promoter Sequence Insertion

Purpose

The Promoter primer is a standard primer that can be used for site directed mutagenesis in all linearized backbones available from the iGEM database and the Anderson RBS sites from the Anderson RBS library.

Background

Most of the BioBrick parts requires a Promoter to express the protein. This has proven problematic as the promoter is very small and hard to insert using 3A Assembly. We believe the creation of an oligo primer with this promoter may be easier to place the promoter in front of the RBS site that is needed to be expressed. An easy oligo primer that could be used in PCR extension and then placed into a vector backbone would save time and frustration as to whether the part had been inserted.