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Team:Warwick/Parts/bb
From 2014.igem.org
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<h1> EXISTING BIOBRICK CHARACTERISATION </h1> <br> <br> | <h1> EXISTING BIOBRICK CHARACTERISATION </h1> <br> <br> | ||
<p> As part of the criterion for the Gold medal, we set about trying to improve the characterisation of an existing part on the registy. In line with the rest of our project, we felt it pertinent that we characterise something relevant and as such we chose <a href="http://parts.igem.org/Part:BBa_J04430">this</a> part. The information below is taken from the registry page:</p> <br> <br> <br> | <p> As part of the criterion for the Gold medal, we set about trying to improve the characterisation of an existing part on the registy. In line with the rest of our project, we felt it pertinent that we characterise something relevant and as such we chose <a href="http://parts.igem.org/Part:BBa_J04430">this</a> part. The information below is taken from the registry page:</p> <br> <br> <br> | ||
| - | < | + | <h4> Introduction</h4> |
<p>Warwick iGEM 2014 used this part as a positive control for our experiments investigating promoter strengths for a self replicating RNA strand, using the Hepatitis C Virus NS5B gene encoding RNA dependent RNA polymerase, as the replicating enzyme.</p> | <p>Warwick iGEM 2014 used this part as a positive control for our experiments investigating promoter strengths for a self replicating RNA strand, using the Hepatitis C Virus NS5B gene encoding RNA dependent RNA polymerase, as the replicating enzyme.</p> | ||
<p>This was measured using a tecan Magellan plate reader in which fluorescence in the GFP range was measured over a period of 24 hours with both biological and technical replicates for each transformed cell type.</p> | <p>This was measured using a tecan Magellan plate reader in which fluorescence in the GFP range was measured over a period of 24 hours with both biological and technical replicates for each transformed cell type.</p> | ||
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Revision as of 00:39, 17 October 2014
EXISTING BIOBRICK CHARACTERISATION
As part of the criterion for the Gold medal, we set about trying to improve the characterisation of an existing part on the registy. In line with the rest of our project, we felt it pertinent that we characterise something relevant and as such we chose this part. The information below is taken from the registry page:
Introduction
Warwick iGEM 2014 used this part as a positive control for our experiments investigating promoter strengths for a self replicating RNA strand, using the Hepatitis C Virus NS5B gene encoding RNA dependent RNA polymerase, as the replicating enzyme.
This was measured using a tecan Magellan plate reader in which fluorescence in the GFP range was measured over a period of 24 hours with both biological and technical replicates for each transformed cell type.
