Team:Nagahama

From 2014.igem.org

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=Abstract=
=Abstract=
:There is the material which is harmful to a creature in the natural world. Cadmium is a kind of the heavy metal in one. We thought to make Escherichia coli having the system which collected harmful cadmium efficiently. We decided to make two kinds of recombination Escherichia coli to complete this system. One has characteristics to release aspartate after addition of cadmium ion, resulting to collect other E. coli. Escherichia coli recognizes aspartic acid as a positive chemotactic factor. The other one can trap cadmium ion outside the bacteria to reduce the influence on cell body.
:There is the material which is harmful to a creature in the natural world. Cadmium is a kind of the heavy metal in one. We thought to make Escherichia coli having the system which collected harmful cadmium efficiently. We decided to make two kinds of recombination Escherichia coli to complete this system. One has characteristics to release aspartate after addition of cadmium ion, resulting to collect other E. coli. Escherichia coli recognizes aspartic acid as a positive chemotactic factor. The other one can trap cadmium ion outside the bacteria to reduce the influence on cell body.
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=Story=
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We make various systems by interaction of cell-cell communication. We keep one function in one E.coli.  This means to make simple plasmid. The following is one example. We’d like to collect cadmium in water. Therefore we use two kinds of E.coli. One catches Cadmium. The other attracts all E.coli by using chemoattractant. Catches E.coli displays metallothionein a protein combines a heavy metal. Cadmium is a kind of heavy metal. The other synthesizes aspartic acid (Asp) one kind of chemoattractant. All E.coli gather in the E.coli synthesizes Asp. To use these E.coli, finally cadmium will be caught.

Revision as of 07:45, 16 October 2014

Abstract

There is the material which is harmful to a creature in the natural world. Cadmium is a kind of the heavy metal in one. We thought to make Escherichia coli having the system which collected harmful cadmium efficiently. We decided to make two kinds of recombination Escherichia coli to complete this system. One has characteristics to release aspartate after addition of cadmium ion, resulting to collect other E. coli. Escherichia coli recognizes aspartic acid as a positive chemotactic factor. The other one can trap cadmium ion outside the bacteria to reduce the influence on cell body.

Story

We make various systems by interaction of cell-cell communication. We keep one function in one E.coli. This means to make simple plasmid. The following is one example. We’d like to collect cadmium in water. Therefore we use two kinds of E.coli. One catches Cadmium. The other attracts all E.coli by using chemoattractant. Catches E.coli displays metallothionein a protein combines a heavy metal. Cadmium is a kind of heavy metal. The other synthesizes aspartic acid (Asp) one kind of chemoattractant. All E.coli gather in the E.coli synthesizes Asp. To use these E.coli, finally cadmium will be caught.