Team:Aachen/Project
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''Cellock Holmes'' encompasses our '''[https://2014.igem.org/Team:Aachen/Project/2D_Biosensor 2D biosensing technology]''' with which we want to enable detection of bacteria on solid surfaces. When developing ''Cellock Holmes'', a main goal was to '''overcome shortcomings''' of excisting detection methods such as high cost, heavy and sensitive equipment, complicated handling and long detection times. Instead of inventing a mainly fancy concept for detecting bacteria, we wanted to create a biosensor that is | ''Cellock Holmes'' encompasses our '''[https://2014.igem.org/Team:Aachen/Project/2D_Biosensor 2D biosensing technology]''' with which we want to enable detection of bacteria on solid surfaces. When developing ''Cellock Holmes'', a main goal was to '''overcome shortcomings''' of excisting detection methods such as high cost, heavy and sensitive equipment, complicated handling and long detection times. Instead of inventing a mainly fancy concept for detecting bacteria, we wanted to create a biosensor that is | ||
- | * '''fast''' | + | #:* '''fast''' |
- | * '''inexpensive''' | + | #:* '''inexpensive''' |
- | * '''open source''' | + | #:* '''open source''' |
- | * '''mobile''' | + | #:* '''mobile''' |
- | * '''easy to handle''' | + | #:* '''easy to handle''' |
As a '''proof-of-concept''' for ''Cellock Holmes'' we decided to detect the bacterium '''''Pseudomonas aeruginosa'''''. This opportunistic bacterium mainly infects patients with open wounds and burns as well as immuneodeficient people. ''P. aeruginosa'' cells use quorum sensing to communicate with each other, and in doing so secrete autoinducers into their environment. Using Synthetic Biology, our team '''engineered sensor cells''', so-called '''''Cellocks''''', that are able to detect said autoinducers and to elicit a fluorescence signal. Furthermore, the response time of our sensor cells is sped up, through the use of our special [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter '''REACh construct''']. | As a '''proof-of-concept''' for ''Cellock Holmes'' we decided to detect the bacterium '''''Pseudomonas aeruginosa'''''. This opportunistic bacterium mainly infects patients with open wounds and burns as well as immuneodeficient people. ''P. aeruginosa'' cells use quorum sensing to communicate with each other, and in doing so secrete autoinducers into their environment. Using Synthetic Biology, our team '''engineered sensor cells''', so-called '''''Cellocks''''', that are able to detect said autoinducers and to elicit a fluorescence signal. Furthermore, the response time of our sensor cells is sped up, through the use of our special [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter '''REACh construct''']. |
Revision as of 15:47, 14 October 2014
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