Team:Aachen/Project
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- | As a '''proof-of-concept''' for ''Cellock Holmes'' we decided to detect the bacterium '''''Pseudomonas aeruginosa'''''. This opportunistic bacterium mainly infects patients with open wounds and burns as well as immuneodeficient people. ''P. aeruginosa'' cells use quorum sensing to communicate with each other, and in doing so secrete autoinducers into their environment. Using Synthetic Biology, our team '''engineered sensor cells, so-called ''Cellocks''''', that are able to detect said autoinducers and to elicit a fluorescence signal. And through the use of our REACh construct, the response time of our sensor cells is sped up. | + | As a '''proof-of-concept''' for ''Cellock Holmes'' we decided to detect the bacterium '''''Pseudomonas aeruginosa'''''. This opportunistic bacterium mainly infects patients with open wounds and burns as well as immuneodeficient people. ''P. aeruginosa'' cells use quorum sensing to communicate with each other, and in doing so secrete autoinducers into their environment. Using Synthetic Biology, our team '''engineered sensor cells, so-called ''Cellocks''''', that are able to detect said autoinducers and to elicit a fluorescence signal. And through the use of our special '''REACh construct''', the response time of our sensor cells is sped up. |
- | So far ''Cellocks'' are able to detect ''P. aeruginosa'' only, however, due to the modular composition of our genetic device, it is possible to engineer ''Cellocks'' in a way that they are able to detect other bacteria's autoinducers. Even more flexibility would be introduced using our alternative molecular approach using Galectin-3. | + | So far ''Cellocks'' are able to detect ''P. aeruginosa'' only, however, due to the modular composition of our genetic device, it is possible to engineer ''Cellocks'' in a way that they are able to detect other bacteria's autoinducers. Even more flexibility would be introduced using our '''alternative molecular approach using Galectin-3'''. |
Hand in hand with the biological side of our project, our IT crew built the '''measurement device ''WatsOn''''' that is able to read and analyze the fluorescent signal. Since we want to make our technology available for everybody, we publish construction manuals and technical details of our device. | Hand in hand with the biological side of our project, our IT crew built the '''measurement device ''WatsOn''''' that is able to read and analyze the fluorescent signal. Since we want to make our technology available for everybody, we publish construction manuals and technical details of our device. |
Revision as of 12:27, 14 October 2014
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