Team:TCU Taiwan/Safety

From 2014.igem.org

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  <legend class="qnum">1. Your Training</legend>
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<p class="qbody"><span class="qnum">a)</span> Have your team members received any safety training yet?</p>
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<li><label for="yes_had_training">Yes, we have already received safety training.</label>
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<p class="qbody"><span class="qnum">b)</span> Please briefly describe the topics that you learned about (or will learn about) in your safety training.</p>
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<p class="qbody"><span class="qnum">c)</span> Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.</p>
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<legend class="qnum">2. Your Local Rules and Regulations</legend>
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<p class="qbody"><span class="qnum">a)</span> Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.</p>
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<p class="qbody"><span class="qnum">b)</span> What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.</p>
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<p class="qbody"><span class="qnum">c)</span> In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.</p>
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<legend class="qnum">3. The Organisms and Parts that You Use</legend>
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<p>Please <a href="https://2014.igem.org/File:TeamName_Safety2014_Spreadsheet.xls" target="_blank">visit this page</a> to download a blank copy of the spreadsheet for question 3. (If you need a CSV version instead of XLS, <a href="https://2014.igem.org/File:TeamName_Safety2014_CSV.txt" target="_blank">visit this page</a>.)</p>
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<p>Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all <strong>new</strong> or <strong>highly modified</strong> protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.</p>
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<p>You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications.</p>
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    <div><button type="button" id="toggle_spreadsheet_instructions">Click here to show/hide instructions for completing the spreadsheet</button></div>
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        <p><strong>Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".</strong></p>
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        <ol style="list-style-type:upper-alpha">
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            <li><strong>Species name (including strain):</strong> For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.</li>
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            <li><strong>Risk Group:</strong> Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)</li>
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            <li><strong>Risk Group Source:</strong> Cite the source from which you obtained the Risk Group information. See <a href="https://2014.igem.org/Safety/Risk_Group_Guide#rghowto">Risk Group Guide</a> for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.</li>
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            <li><strong>Disease risk to humans?:</strong> Does this organism cause any disease in humans? If yes, what disease does it cause?</li>
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            <li><strong>Part number/name:</strong> For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.</li>
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            <li><strong>Natural function of part:</strong> For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.</li>
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            <li><strong>How did you acquire it?:</strong> Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)</li>
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            <li><strong>How will you use it?:</strong> Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")</li>
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            <li><strong>Notes:</strong> Use this column to give any additional information that is necessary.</li>
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<p><strong><a href="https://2014.igem.org/wiki/index.php?title=Special:Upload&amp;wpDestFile=TCU_Taiwan_Safety2014_Spreadsheet.xls" target="_blank" id="spreadsheet_upload"><button type="button">Upload Spreadsheet</button></a> -- Please do not change the "Destination Filename"!</strong></p>
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<p>You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order.</p>
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<p><strong><a href="https://2014.igem.org/File:TCU_Taiwan_Safety2014_Spreadsheet.xls" target="_blank" id="spreadsheet_view">Click here to VIEW your spreadsheet</a></strong></p>
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<legend class="qnum">4. Risks of Your Project Now</legend>
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<p class="qbody">Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.</p>
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<p class="qbody"><span class="qnum">a)</span> Risks to the safety and health of team members, or other people working in the lab:</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="lab_worker_risks_now" data-form-field="lab_worker_risks_now" readonly></textarea></div>
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<p class="qbody"><span class="qnum">b)</span> Risks to the safety and health of the general public (if any biological materials escaped from your lab):</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="public_risks_now" data-form-field="public_risks_now" readonly></textarea></div>
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<p class="qbody"><span class="qnum">c)</span> Risks to the environment (from waste disposal, or from materials escaping from your lab):</p>
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<p class="qbody"><span class="qnum">d)</span> Risks to security through malicious mis-use by individuals, groups, or countries:</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="security_now" data-form-field="security_now" readonly></textarea></div>
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<p class="qbody"><span class="qnum">e)</span> What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="reduce_risks_now" data-form-field="reduce_risks_now" readonly></textarea></div>
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<legend class="qnum">5. Risks of Your Project in the Future</legend>
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<p><strong>What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.</strong></p>
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<p class="qbody"><span class="qnum">a)</span> What <i>new</i> risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the <i>knowledge</i> you generate or the <i>methods</i> you develop became widely available?</p>
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<p class="qbody"><span class="qnum">b)</span> Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="4" id="minimize_future_risks" data-form-field="minimize_future_risks" readonly></textarea></div>
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<legend class="qnum">6. Further Comments</legend>
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<p class="qbody">If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank.</p>
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<p class="qbody">You can also use this space for any other comments or additional material.</p>
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<div class="wrapper" style="background-color: white;"><textarea rows="5" id="further_comments" data-form-field="further_comments" readonly></textarea></div>
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<legend><h4>Submit</h4></legend>
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<p>Only a team Instructor (or Primary Contact) may submit the Safety Form.</p>
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<p>Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.</p>
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   <td colspan="3" height="1px" align="center" width="70%"><strong><font face="Trebuchet MS" size="6" color="#A66B38"><p>TCU_Taiwan iGEM Laboratory Practices</p></font></strong>
   <td colspan="3" height="1px" align="center" width="70%"><strong><font face="Trebuchet MS" size="6" color="#A66B38"><p>TCU_Taiwan iGEM Laboratory Practices</p></font></strong>

Revision as of 16:47, 10 October 2014


 
Safety
  
Highest Code
 
  • ENJOY THE iGEM!
  • All team member has to take lab safety training.
    •
  • No eating in the lab.

 

 
1. Your Training

a) Have your team members received any safety training yet?




b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.



c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.


2. Your Local Rules and Regulations

a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.



b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.



c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.


3. The Organisms and Parts that You Use

Please visit this page to download a blank copy of the spreadsheet for question 3. (If you need a CSV version instead of XLS, visit this page.)

Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.

You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications.



-- Please do not change the "Destination Filename"!

You may upload multiple versions of your spreadsheet, using the same Destination Filename. The wiki software will keep track of different versions, and list them in chronological order.

Click here to VIEW your spreadsheet


4. Risks of Your Project Now

Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.


a) Risks to the safety and health of team members, or other people working in the lab:



b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):



c) Risks to the environment (from waste disposal, or from materials escaping from your lab):



d) Risks to security through malicious mis-use by individuals, groups, or countries:



e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)


5. Risks of Your Project in the Future

What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.


a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?



b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.) Such features are not required for an iGEM project, but many teams choose to explore them.


6. Further Comments

If you are completing a Preliminary Version of your Safety Form, use this space to describe how far you have progressed in your project, and give some comments about any questions that you left blank.

You can also use this space for any other comments or additional material.


Submit

Only a team Instructor (or Primary Contact) may submit the Safety Form.

Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.

    

We need to go back and edit our answers --

Delete Form (admins only)

TCU_Taiwan iGEM Laboratory Practices
   
1. Don’t eat, drink, prepare or store food, smoke, handle contact lenses or apply cosmetics in any laboratory.
2. Know where the nearest eyewash, safety shower, and fire extinguisher are located. Know how to use them.
3. Insist upon good housekeeping in your laboratory.
4. Check for insects and rodents. Keep all areas clean.
5. Secure all gas cylinders.
6. Wear laboratory coats and other appropriate protective clothing while performing laboratory activities. Feet and legs should be covered; sandals and open-toed shoes should not be worn in laboratories. Wear appropriate gloves while handling infectious or toxic materials and animals. Do not wear lab coats. Gloves or other personal protective equipment outside the laboratory.
7. Use a biological safety cabinet for handling infectious materials or materials requiring protection from contamination and a fume hood for toxic materials; mixed hazards need to be evaluated case by case.
8. Fume hoods should be used for laboratory activities that could result in chemical explosions or fires, for experiments involving toxic, hazardous or carcinogenic compounds, and use of strong acids and bases. Biological safety cabinets should not be used for this kind of work. Fume hoods are workstations, not storage cabinets. Vented storage areas may be located under the fume hood work area. However, these are not for storage of flammables.
9. Respect chemicals and radionuclides. Know their hazards and follow appropriate safety precautions. Chemical and radioactive waste must not be poured down the drain.
10. All equipment must be documented to be free of chemical, biological, and radiological contamination before repair work is done or before moving equipment for storage or elsewhere.
11. Never mouth pipette anything. Use mechanical pipetting devices only!
12. Close laboratory doors while experiments are in progress. Restrict access to laboratory.
13. Put liquid traps and in-line vacuum filters on all vacuum lines.
14. Minimize or contain all aerosol-producing activities, large-volume work, concentrated solutions or cultures. These activities include centrifugation (use safety cups), vortex missing (stopper tube), blending (use metal safety blender), sonication, grinding, opening containers of infectious material, inoculating culture flasks, inoculating animals, harvesting infectious materials from cultures or animals, and weighing or reconstructing toxic powders, etc.
15. Place biological safety cabinets in low-traffic areas and minimize activities that disrupt air flow in or around cabinet.
16. Decontaminate all work surfaces daily, and decontaminate all spills immediately.
17. Decontaminate (by autoclaving or chemical disinffection) all biologically contaminated materials – glassware, animal cages, laboratory equipment, etc. – before washing, reuse or disposal. Discard materials via proper waste stream.
18. Be careful with needles and syringes. Use only when alternative methods are not feasible.
19. Syringes, needles, Pasteur pipettes, etc, should be placed in rigid, leak-proof containers (Sharps Safe) and discarded following the waste rules.
20. Broken glassware and disposable pipettes (after decontamination) should be placed in a “Disposable Labware and Broken Glass Box” and discarded following the waste rules.
21. Place contaminated biological materials in covered, leak-proof containers before removing them from the laboratory.
22. Wash your hands after handling chemicals, infectious materials, animals, after removing gloves and before leaving the laboratory.

Welcome!

Timeline

Visit the Safety Hub to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.


Edit this page!

Please use this page to write about anything related to safety in your project.

Your Lab

Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space!

  • Now : Read the Safety Hub and learn about safety in iGEM. Ask questions by emailing safety at igem DOT org .
  • Now - Jamboree: Complete Check-Ins and receive approval before acquiring and using certain materials in your lab
  • Now - Wiki Freeze: Edit this Safety page to tell us about what you're doing
  • June 9: Submit the About Our Lab form.
  • Let us know by June 25 if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.
  • June 30: Submit the Preliminary Version of the Safety Form.
  • Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).
  • September 1: Submit the Final Version of the Safety Form.
  • October: Wiki freeze (exact date to be determined)
  • October 30 - November 3: GIANT JAMBOREE!
health worker    
     
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