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| - | <h1>Materials</h1> | + | <h1> 1.Search and Find Anti-Cancer Genes </h1> |
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| - | <!-- Inhaltsverzeichnis-->
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| - | <h2>1. Chemicals </h2>
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| - | <br>
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| | | | |
| - | {|
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| - | |+
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| - | ! Chemical
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| - | ! Manufacturer
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| - | |-
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| - | | 2 log DNA ladder
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| - | | New England Biolabs
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| - | |-
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| - | | 2-Mercapto Ethanol
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| - | | Merck
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| - | |-
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| - | | Acetic Acid 100%
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| - | | zChL UdS
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| - | |-
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| - | | Agar Kobe I
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| - | | Roth
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| - | |-
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| - | | Agarose
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| - | | Peqlab, Biozym
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| - | |-
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| - | | Ampicillin
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| - | | Roth
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| - | |-
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| - | | APS (Ammoniumpersulfat)
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| - | | zChL UdS
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| - | |-
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| - | | BactoTM Casamino-acids
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| - | | BD
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| - | |-
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| - | | BactoTM Tryptone
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| - | | BD
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| - | |-
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| - | | BactoTM Yeast-Extract
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| - | | BD
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| - | |-
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| - | | Boric Acid
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| - | | Merck
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| - | |-
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| - | | Bovine Serum Albumin (BSA)
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| - | | New England Biolabs
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| - | |-
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| - | | Bromophenol Blue
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| - | | Merck
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| - | |-
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| - | | Calcium Chloride
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| - | | Calbiochem
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| - | |-
| |
| - | | Chloramphenicol
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| - | | Fagran
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| - | |-
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| - | | D(+)-Glucose
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| - | | Sigma Aldrich
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| - | |-
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| - | | ddH2O (MilliQ) (MILLIPORE 0,22 μm Membranfilter)
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| - | | Biocel
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| - | |-
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| - | | DifcoTM Antibiotic Medium No. 3
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| - | | BD
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| - | |-
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| - | | Dimethylsulfoxide (DMSO)
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| - | | Sigma Aldrich
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| - | |-
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| - | | dNTP-Mix (10 mM)
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| - | | Peqlab
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| - | |-
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| - | | EDTA
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| - | | Roth
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| - | |-
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| - | | Ethanol, HPLC grade
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| - | | Sigma Aldrich
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| - | |-
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| - | | Glutamate
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| - | | Sigma Aldrich
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| - | |-
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| - | | Glycerol
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| - | | zChL UdS
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| - | |-
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| - | | Hydrochloric Acid 37 % (HCl)
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| - | | VWR
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| - | |-
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| - | | Immersion Oil
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| - | | Leica
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| - | |-
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| - | | Isopropanol
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| - | | Sigma Aldrich
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| - | |-
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| - | | LB-Medium (Miller)
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| - | | Sigma Aldrich
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| - | |-
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| - | | Magnesium Chloride Hexahydrate
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| - | | VWR
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| - | |-
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| - | | Magnesium Sulfate
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| - | | zChL UdS
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| - | |-
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| - | | Maleic Acid
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| - | | Roth
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| - | |-
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| - | | Natriumchlorid
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| - | | VWR
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| - | |-
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| - | | Natriumhydroxid
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| - | | Grüssing
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| - | |-
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| - | | PEG-6000
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| - | | Merck
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| - | |-
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| - | | peqGREEN
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| - | | Peqlab
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| - | |-
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| - | | Potassium Chloride
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| - | | Grüssing
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| - | |-
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| - | | Potassium Dihydrogen Orthophosphate
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| - | | Grüssing
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| - | |-
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| - | | Potassium Hydrogen Phosphate
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| - | | Grüssing
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| - | |-
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| - | | Potassium Sulfate
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| - | | zChL UdS
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| - | |-
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| - | | Proline
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| - | | Merck
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| - | |-
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| - | | Protein Standard
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| - | | New England Biolabs
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| - | |-
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| - | | Rotiphorese Gel 30
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| - | | Roth
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| - | |-
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| - | | SDS
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| - | | Applichem/Roth
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| - | |-
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| - | | Succrose
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| - | | Sigma Aldrich
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| - | |-
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| - | | Temed
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| - | | Applichem
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| - | |-
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| - | | Tetracycline
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| - | | Serva
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| - | |-
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| - | | Tris-Baze
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| - | | Sigma Aldrich
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| - | |-
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| - | | Trizma Base
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| - | | Sigma Aldrich
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| - | |-
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| - | | Xylose
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| - | | Sigma Aldrich
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| - | |-
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| - | |}
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| - | <br>
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| - | <h2> 2. Enzymes</h2>
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| - | <br>
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| - | {|
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| - | |+
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| - | !Enzyme
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| - | !Manufacturer
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| - | |-
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| - | | Lysozyme
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| - | | Serva
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| - | |-
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| - | | T4 Ligase
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| - | | Thermo Scientific
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| - | |-
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| - | | Taq-Polymerase
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| - | | New England Biolabs
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| - | |-
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| - | | Q5 High Fidelity Polymerase (Master Mix)
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| - | | New England Biolabs
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| - | |-
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| - | | FastDigest <i>Mlu</i>I
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| - | | Thermo Scientific
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| - | |-
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| - | | FastDigest <i>Sac</i>I
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| - | | Thermo Scientific
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| - | |-
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| - | | FastDigest <i>Sph</i>I
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| - | | Thermo Scientific
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| - | |-
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| - | | FastDigest <i>Eco</i>RI
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| - | | Thermo Scientific
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| - | |-
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| - | | FastDigest <i>Xba</i>I
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| - | | Thermo Scientific
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| - | |-
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| - | | FastDigest <i>Spe</i>I
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| - | | Thermo Scientific
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| - | |-
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| - | | FastDigest <i>Pst</i>I
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| - | | Thermo Scientific
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| - | |-
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| - | | Fast AP
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| - | | Thermo Scientific
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| - | |-
| |
| - | |}
| |
| - | <br>
| |
| | | | |
| - | <h2> 3. oligonucleotides </h2>
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| - | <br>
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| - | {|
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| - | |+
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| - | !Abbreviation
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| - | !5´-Sequence-3´
| |
| - | |-
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| - | | <b>Generation of <i>has2-gfp</i> fusion gene</b>
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| - | |
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| - | |-
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| - | | SOE Has for:
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| - | | TGTACACGCGTAAATCAAGGAGGTG
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| - | |-
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| - | | SOE Has rev:
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| - | | CTCGCCCTTGCTCACCATTACATCTAATACCATATCGTATTGTTGACC
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| - | |-
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| - | | SOE GFP for:
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| - | | ATGGTATTAGATGTAATGGTGAGCAAGGGCGAG
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| - | |-
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| - | | SOE GFP rev:
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| - | | TGTACGAGCTCTTACTTGTACAGCTCGTCCATGC
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| - | |-
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| - | |<b>Amplification of genomic DNA of <i>B. megaterium</i></b>
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| - | |
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| - | |-
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| - | | RBS UDP-GlcDH for
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| - | | TGTACGAGCTCAAATCAAGGAGGTGAATATACAATGACTAATATCACAGTAGCGGGTACTGG
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| - | |-
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| - | | UDP-GlcDH rev:
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| - | | TGTACGCATGCTTAGACATTAGCTTTAATTTTAGCTTTGTATCC
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| - | |-
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| - | | Glk for
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| - | | TGTAC GAATTC ATGGATGACAAATGGTTAG
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| - | |-
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| - | | Glk rev:
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| - | | TGTAC CTGCAG TTA TTATTCAAAATAAGAT
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| - | |-
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| - | | PgmB for
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| - | | TGTAC GAATTC ATGAAAAAAGAAGAGCTAC
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| - | |-
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| - | | PgmB rev:
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| - | |TGTAC ACTAGT TTA TTAATTTTTTGTATAC
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| - | |-
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| - | | GtaB for
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| - | | TGTAC GAATTC ATGACGATAAAAAAGGCAG
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| - | |-
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| - | | GtaB rev:
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| - | | TGTAC CTGCAG TTA TTAGCTGAAGTTGTTTGC
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| - | |-
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| - | | Pgi for
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| - | | TGTAC TCTAGA ATGACACATGTTCGTTTTG
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| - | |-
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| - | | Pgi rev:
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| - | | TGTAC CTGCAG TTA TTATTTCAAACGCTTTTC
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| - | |-
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| - | | GlmS for
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| - | | TGTAC TCTAGA ATGTGCGGAATTGTAGG
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| - | |-
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| - | | GlmS rev:
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| - | | TGTAC CTGCAG TTA TTATTCTACAGTAACTG
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| - | |-
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| - | | GlmM for
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| - | | TGTAC GAATTC ATGGGTAAGTATTTTGG
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| - | |-
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| - | | GlmM rev:
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| - | | TGTAC CTGCAG TTA TTACTCTAAGCCCATTTC
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| - | |-
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| - | | GcaD for
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| - | | TGTAC GAATTC ATGTCAAAAAGATATGC
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| - | |-
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| - | | GcaD rev:
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| - | | TGTAC CTGCAG TTA TTAGGATTTTTTATTAATATC
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| - | |-
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| - | |<b>Quick Change PCR for restriction site mutagenesis</b>
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| - | |
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| - | |-
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| - | | QC Has for:
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| - | | GAATTTGTAGAAGATTGGTACTCTCAAGAATTTATGGGTAACCAG
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| - | |-
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| - | | QC has rev:
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| - | | AAATTCTTGAGAGTACCAATCTTCTACAAATTCGTGTAATAAAGAG
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| - | |-
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| - | | QC UDP-GlcDH for:
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| - | | TAATTATTAAAAACTCTGATGCTGGTAAATTATTTTTCACAACTG
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| - | |-
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| - | | QC UDP-GlcDH rev:
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| - | | ACCAGCATCAGAGTTTTTAATAATTAATTCATCAAGCCCAGG
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| - | |-
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| - | | QC PgmB for:
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| - | | TTGCAGCTGCTGATTGAGTTCTTCAG
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| - | |-
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| - | | QC PgmB rev:
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| - | | CTGCAAGGGATCAGCCGAGTG
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| - | |-
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| - | | QC Pgi for:
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| - | | AAATTCTTCTTTGTCATACTCCGTAGG
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| - | |-
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| - | | QC Pgi rev:
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| - | | GAATTTGCACGCATTCAAAAAAGTGC
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| - | |-
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| - | | QC GlmS for:
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| - | | AAATTCACTTGCTACGTGCACTTCTAC
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| - | |-
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| - | | QC GlmS rev:
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| - | | GAATTTTCTTATAACATGCCGCTTTTATCTG
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| - | |-
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| - | | QC GlmM for:
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| - | | TCGAGATCTGCAAATAAATGTGCTGC
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| - | |-
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| - | | QC GlmM for:
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| - | | TCTCGATGCAGATATCTCTACAATGG
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| - | |-
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| - | |<b>Sequencing</b>
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| - | |
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| - | |-
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| - | | pSB1C3 check for:
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| - | | TGCCACCTGACGTCTAAGAAACC
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| - | |-
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| - | | pSB1C3 check rev:
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| - | | ATTACCGCCTTTGAGTGAGCTG
| |
| - | |-
| |
| - | |}
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| - | <br>
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| - | <h2> 4. Media and Solutions </h2>
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| - | <br>
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| - | {|
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| - | |+
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| - | !Solution
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| - | !Ingredients
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| - | !Quantity
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| - | |-
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| - | |<b>10 x KPP Buffer</b>
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| - | |KH₂PO₄<br>
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| - | K₂HPO₄<br>
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| - | to 100 ml ddH2O<br>
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| - | <b>autoclaved</b>
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| - | |2,71 g<br>
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| - | 12.5 g <br> <br><br>
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| - | |-
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| - | |<b>Stacking Gel Buffer</b>
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| - | |Tris<br>
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| - | SDS<br>
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| - | Adjust pH value to 6.7
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| - | to 100 ml ddH2O
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| - | |6.05 g<br>
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| - | 0.4 g<br><br>
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| - | |-
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| | | | |
| - | |<b>Stacking Gel (4 %)</b>
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| - | |ddH2O<br>
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| - | Stacking Gel Buffer<br>
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| - | Rotiphorese Gel 30<br>
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| - | Temed<br>
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| - | 10% APS<br>
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| - | |1193 µl<br>
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| - | 500 µl <br>
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| - | 267 µl<br>
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| - | 5 µl<br>
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| - | 10 µl<br>
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| - | |-
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| - | |<b>Separating Gel Buffer</b>
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| - | |Tris<br>
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| - | SDS<br>
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| - | Adjust pH value to 8.8
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| - | To 100 ml ddH2O
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| - | |18.17 g<br>
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| - | 0.4 g<br>
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| - | <br>
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| - | |-
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| - | |<b>Separating Gel (12%)</b>
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| - | |ddH2O<br>
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| - | Separating Gel Buffer<br>
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| - | Rotiphorese Gel 30<br>
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| - | Temed<br>
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| - | 10% APS<br>
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| - | |1350 µl<br>
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| - | 1000 µl<br>
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| - | 1600 µl<br>
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| - | 10 µl<br>
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| - | 20 µl<br>
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| - | |-
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| - | |<b>5 x SDS Sample Buffer</b>
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| - | |Tris <br>
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| - | 2-ME<br>
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| - | SDS <br>
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| - | Glycerol<br>
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| - | Bromophenol Blue<br>
| |
| - | Adjust pH value to 6.5 with HCl
| |
| - | to 50 ml ddH2O
| |
| - | |1.21 g<br>
| |
| - | 3.487 g<br>
| |
| - | 5 g<br>
| |
| - | 10 ml<br>
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| - | 25 mg<br> <br>
| |
| - | |-
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| - | |<b>10 x TBE</b>
| |
| - | |Trizma-Baze<br>
| |
| - | Boric Acid<br>
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| - | EDTA<br>
| |
| - | to 1000 ml ddH2O
| |
| - | |162 g<br>
| |
| - | 27.5 g<br>
| |
| - | 9.3 g<br><br>
| |
| - | |-
| |
| - | |<b>12 % Proline</b>
| |
| - | |Proline<br>
| |
| - | to 25 ml ddH2O<br>
| |
| - | <b>sterile filtered</b>
| |
| - | |3 g <br> <br><br><br>
| |
| - | |-
| |
| - | |<b>2 x AB3</b>
| |
| - | |Antibiotic Medium NO. 3 (Difco)<br>
| |
| - | to 200 ml ddH2O<br>
| |
| - | <b>autoclaved</b>
| |
| - | |7 g <br> <br> <br><br>
| |
| - | |-
| |
| - | |<b>2 x SMM</b>
| |
| - | |Maleic Acid<br>
| |
| - | NaOH<br>
| |
| - | MgCl₂ x 6H2O<br>
| |
| - | Succrose<br><br>
| |
| - | Solve in 150 ml ddH2O<br>
| |
| - | Adjust pH value to 6.5 with HCl to 250 ml ddH2O.<br>
| |
| | | | |
| - | <b>sterile filtered</b>
| |
| - | |1.16 g<br>
| |
| - | 0,8 g<br>
| |
| - | 2.03 g<br>
| |
| - | 85.58 g<br>
| |
| - | <br> <br>
| |
| - | <br><br> <br>
| |
| - | |-
| |
| - | |<b>20 % Glucose</b>
| |
| - | |Glucose<br>
| |
| - | to100 ml ddH2O<br>
| |
| - | <b>steril filtered</b>
| |
| - | |20 g<br> <br> <br><br>
| |
| - | |-
| |
| - | |<b>3.5 ml CR5-Topagar-Portionen</b>
| |
| - | |Solution A<br>
| |
| - | Solution B<br>
| |
| - | 8 x CR5-Salts<br>
| |
| - | 12 % Proline<br>
| |
| - | 20 % Glucose
| |
| - | |1.75 ml<br>
| |
| - | 1 ml<br>
| |
| - | 0.346 ml<br>
| |
| - | 0.175 ml<br>
| |
| - | 0.175 ml<br>
| |
| - | |-
| |
| - | |<b>8 x CR5-Salze </b>
| |
| - | |K₂SO₄<br>
| |
| - | KH₂PO₄<br>
| |
| - | MgCl₂ x 6H₂O<br>
| |
| - | CaCl₂<br>
| |
| - | to 625 ml ddH2O<br>
| |
| - | <b>autoclaved</b>
| |
| - | |1.25 g<br>
| |
| - | 0.25 g <br>
| |
| - | 50 g <br>
| |
| - | 11 g<br><br>
| |
| - | <br>
| |
| - | |-
| |
| - | |<b> Loading Dye for Agarose Gels </b>
| |
| - | |25 mM Tris-HCl<br>
| |
| - | Adjust pH value to 7.8 with HCl<br>
| |
| - | Bromophenol Blue<br>
| |
| - | Xylene Cyanol<br>
| |
| - | Glycerol<br>
| |
| - | EDTA<br>
| |
| - | |4 ml<br>
| |
| - | <br>
| |
| - | 3 mg<br>
| |
| - | 3 mg<br>
| |
| - | 6 ml<br><br>
| |
| - | |-
| |
| - | |<b>Ampicillin Stock Solution (100 mg/ml)</b>
| |
| - | |Ampicillin<br>
| |
| - | Ethanol<br>
| |
| - | ddH2O
| |
| - | |1000 mg<br>
| |
| - | 5ml<br>
| |
| - | 5ml<br>
| |
| - | |-
| |
| - | |<b> Chloramphenicol Stock Solution (10 mg/ml)</b>
| |
| - | |Chloramphenicol<br>
| |
| - | to 10 ml Ethanol
| |
| - | |100mg <br> <br>
| |
| - | |-
| |
| - | |<b> CR5-Topagar</b>
| |
| - | |<b>SolutionA:</b>
| |
| - | Succrose<br>
| |
| - | MOPS<br>
| |
| - | NaOH<br>
| |
| - | to 250 ml ddH2O<br>
| |
| - | Adjust pH value to 7.3 with NaOH<br>
| |
| - | <b>Steril filtered</b> <br><br>
| |
| - | <b>Solution B:</b> <br>
| |
| - | Agar<br>
| |
| - | Casamino Acids (Difco)<br>
| |
| - | Yeast Extract<br>
| |
| - | to 142.5 ml ddH2O<br>
| |
| - | <b>autoclaved</b>
| |
| - | |<br><br>
| |
| - | 51.5 g<br>
| |
| - | 3.25 g<br>
| |
| - | 0.3 g<br><br> <br> <br> <br><br>
| |
| - | 2 g<br>
| |
| - | 0.1 g<br>
| |
| - | 5 g <br> <br> <br>
| |
| - | |-
| |
| - | |<b> LB-Agar </b>
| |
| - | |LB-Medium (Sigma Aldrich)<br>
| |
| - | Agar<br>
| |
| - | to 400 ml ddH2O<br>
| |
| - | <b>autoclaved</b>
| |
| - | |10 g
| |
| - | 6 g
| |
| - | <br>
| |
| - | <br><br>
| |
| - | |-
| |
| - | |<b> LB-Medium </b>
| |
| - | |LB-Medium (Sigma Aldrich)<br>
| |
| - | to 400 ml ddH2O<br>
| |
| - | <b>autoclaved</b>
| |
| - | |12 g <br> <br> <br> <br>
| |
| - | |-
| |
| - | |<b> PEG-P </b>
| |
| - | |PEG-6000<br>
| |
| - | 1 x SMM<br>
| |
| - | <b>autoclaved</b>
| |
| - | |20 g<br>
| |
| - | 50 ml<br><br>
| |
| - | |-
| |
| - | |<b>SMMP</b>
| |
| - | |2 x AB3<br>
| |
| - | 2 x SMM
| |
| - | |25ml<br>
| |
| - | 25 ml<br>
| |
| - | |-
| |
| - | |<b> SOC-Medium </b>
| |
| - | |<b>Solution 1:</b> <br>
| |
| - | Bacto Tryptone<br>
| |
| - | Yeast Extrakt<br>
| |
| - | 1 M KCl<br>
| |
| - | 1 M MgCl₂<br>
| |
| - | 1 M MgSO₄<br>
| |
| - | 5 M NaCl<br>
| |
| - | to 990 ml ddH2O<br>
| |
| - | <b>autoclaved</b> <br> <br>
| |
| - | <b>Solution 2:</b><br>
| |
| - | 40 % Glucose (w/v)<br>
| |
| - | <b>sterile filtered</b><br> <br>
| |
| - | Add Solution 1 to Solution 2
| |
| - | | <br>
| |
| - | 20 g<br>
| |
| - | 5 g<br>
| |
| - | 2,5 ml<br>
| |
| - | 10 ml<br>
| |
| - | 10 ml<br>
| |
| - | 2 ml<br> <br><br> <br>
| |
| - | 10 ml<br>
| |
| - | <br> <br> <br> <br>
| |
| - | |-
| |
| - | | <b> TB-Medium </b>
| |
| - | |Yeast Extrakt<br>
| |
| - | Tryptone<br>
| |
| - | Glycerol<br>
| |
| - | to 900 ml ddH2O<br>
| |
| - | <b>autoclaved</b> <br>
| |
| - | Add 100 ml 10 x KPP Buffer
| |
| - | |24 g<br>
| |
| - | 12 g<br>
| |
| - | 4 ml<br> <br> <br> <br>
| |
| - | |-
| |
| - | |<b> Tetracyclin Stammlösung (10 mg/ml) </b>
| |
| - | |Tetracycline<br>
| |
| - | to 10 ml Ethanol
| |
| - | |100 mg <br> <br> <br>
| |
| - | |-
| |
| - | |<b> Xylose Solution (250 mg/ml)</b>
| |
| - | | Xylose<br>
| |
| - | to 10 ml ddH2O
| |
| - | |2.5 g <br> <br> <br>
| |
| - | |-
| |
| - | |}
| |
| - | <br>
| |
| | | | |
| - | <h2> 5. Equipment </h2>
| |
| - | <br>
| |
| - | {|
| |
| - | |+
| |
| - | !Equipment
| |
| - | !Manufacturer
| |
| - | |-
| |
| - | |300 ml Erlenmeyer Flask
| |
| - | |Schott Duran
| |
| - | |-
| |
| - | |300 ml Baffled Flask
| |
| - | |Thermo Scientific
| |
| - | |-
| |
| - | |2000 ml Erlenmeyer Flask
| |
| - | |Schott Duran
| |
| - | |-
| |
| - | |Ultra Low Temoerature Freezer
| |
| - | |Heraeus
| |
| - | |-
| |
| - | |Freezer
| |
| - | |Sigma Aldrich
| |
| - | |-
| |
| - | |Refrigerator
| |
| - | |Liebherr
| |
| - | |-
| |
| - | |Autoclave: Systec VX-150
| |
| - | |Systec
| |
| - | |-
| |
| - | |Cover Slips
| |
| - | |Roth
| |
| - | |-
| |
| - | |Ice Machine
| |
| - | |Ziegra
| |
| - | |-
| |
| - | |Electroporation Cuvettes (gap width 0.1 cm)
| |
| - | |Sigma-Aldrich
| |
| - | |-
| |
| - | |Elektroporator: Easyject Prima
| |
| - | |EquiBio
| |
| - | |-
| |
| - | |<b>Falcon Tubes:</b> <br>
| |
| - | 15 ml <br>
| |
| - | 50 ml<br>
| |
| - | |Sarstedt
| |
| - | |-
| |
| - | |<b>Electrophoresis systems:</b> <br>
| |
| - | PowerPACTM High-Current Power Supply<br>
| |
| - | Mini PROTEANTM 3 Cell<br>
| |
| - | PerfectBlue Gel System Mini S
| |
| - | |<br>
| |
| - | Biorad<br>
| |
| - | Biorad<br>
| |
| - | Peqlab<br>
| |
| - | |-
| |
| - | | <b>Western Blot System:</b> <br>
| |
| - | Blotting Device: OwlTM Separation System<br>
| |
| - | Power Supply: Voltcraft DPS-4005PFC
| |
| - | | <br>
| |
| - | Thermo Fisher Scientific<br>
| |
| - | Conrad Electronic<br>
| |
| - | |-
| |
| - | |<b>Fluorescence microscopy:</b> <br>
| |
| - | Inverse Fluorescence microscope Axiovert 200M<br>
| |
| - | CCD Digital camera Coolsnap HQ2
| |
| - | |<br>
| |
| - | Zeiss<br>
| |
| - | Photometrics<br>
| |
| - | |-
| |
| - | |Scale (MXX2001)
| |
| - | |Denver Instruments
| |
| - | |-
| |
| - | |Micro scale (BP 210 S)
| |
| - | |Sartorius
| |
| - | |-
| |
| - | | <b>Gloves:</b> <br>
| |
| - | Nitrile<br>
| |
| - | Latex
| |
| - | |VWR
| |
| - | |-
| |
| - | |Imaging System Chemi DOC XRS+
| |
| - | |Biorad
| |
| - | |-
| |
| - | |Incubator: Innova 4230
| |
| - | |Innova
| |
| - | |-
| |
| - | |<b>Cuvettes:</b> <br>
| |
| - | Polystyrol/Polystyrene 10 x 4x 45 mm<br>
| |
| - | Quarz
| |
| - | |Sarstedt
| |
| - | |-
| |
| - | |Magnetic Mixer IKAMAG® RCT
| |
| - | |IKA® Labortechnik
| |
| - | |-
| |
| - | |Microliter Pipettes:
| |
| - | |Brand
| |
| - | |-
| |
| - | |Microliter Pipette Tips:
| |
| - | |Sarstedt
| |
| - | |-
| |
| - | |Microwave: Severin 700
| |
| - | |Severin
| |
| - | |-
| |
| - | |Microscope slides
| |
| - | |Roth
| |
| - | |-
| |
| - | |<b>PCR-Cycler: </b> <br>
| |
| - | Primus advanced 96
| |
| - | |Peqlab
| |
| - | |-
| |
| - | |pH-Meter: 766 Calimatic
| |
| - | |Knick
| |
| - | |-
| |
| - | |Photometer: UV2100 UV/VIS Recording Spectrophotometer
| |
| - | |Shimadzu
| |
| - | |-
| |
| - | |Petri Dishes
| |
| - | |Sarstedt
| |
| - | |-
| |
| - | |Reusable Glass Media Bottles with Cap
| |
| - | |Fisher Scientific
| |
| - | |-
| |
| - | |<b>Reaction Tubes:</b> <br>
| |
| - | 1,5 ml<br>
| |
| - | 2 ml<br>
| |
| - | PCR-Softtubes
| |
| - | |Sarstedt
| |
| - | |-
| |
| - | |Speedvac
| |
| - | |UNIVAPO 100H
| |
| - | |-
| |
| - | |Thermomixer 5436
| |
| - | |Eppendorf
| |
| - | |-
| |
| - | |Compartment Dryer
| |
| - | |Memmert
| |
| - | |-
| |
| - | |Mini UV-Table (60-ECX-20M)
| |
| - | |Peqlab
| |
| - | |-
| |
| - | |Water Bath SUB6 Grant
| |
| - | |Biolab
| |
| - | |-
| |
| - | |Vortex Genie 2
| |
| - | |Bender & Hobein
| |
| - | |-
| |
| - | |<b>Centrifuges:</b> <br>
| |
| - | Biofuge pico<br>
| |
| - | Multifuge 1L
| |
| - | |Heraeus
| |
| - | |-
| |
| - | |}
| |
| - | <br>
| |
| - |
| |
| - | <h2> 6. Kits </h2>
| |
| - | <br>
| |
| - | {|
| |
| - | |+
| |
| - | !Kit
| |
| - | !Manufacturer
| |
| - | |-
| |
| - | |QIAprep Spin Miniprep Kit
| |
| - | |Qiagen
| |
| - | |-
| |
| - | |QIAquick Gel Extraction Kit
| |
| - | |Qiagen
| |
| - | |-
| |
| - | |Gibson Assembly TM Cloning Kit
| |
| - | |NEB
| |
| - | |-
| |
| - | |DNA Distribution Kit 2014
| |
| - | |iGEM
| |
| - | |-
| |
| - | |Nexttec TM 1-Step DNA Isolation Kit for Bacteria
| |
| - | |NexttecTM
| |
| - | |-
| |
| - | |}
| |
| - | <br>
| |
| | | | |
| - | <h2> 7. Strains </h2>
| |
| - | <br>
| |
| - | {|
| |
| - | |+
| |
| - | !Bakterienstamm
| |
| - | !Genotyp
| |
| - | !Referenz
| |
| - | |-
| |
| - | | <i>E. coli</i> TOP10 F`
| |
| - | |F'{lacIqTn10(TetR)} mcrA ∆(mrr-hsdRMS-mcrBC) <br>
| |
| - | Φ80lacZ∆M15 ∆lacX74 recA1 araD139 ∆(ara-leu)<br>
| |
| - | 7697 galU galK rpsL endA1 nupG
| |
| - | |Invitrogen
| |
| - | |-
| |
| - | |<i>B. megaterium</i> MS941
| |
| - | |ΔnprM
| |
| - | |Wittchen et al 1995
| |
| - | |-
| |
| - | |}
| |
| | | | |
| | | | |
| | </div> | | </div> |
| | </div> | | </div> |
"
