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Team:KIT-Kyoto/Notebook/Labnote
From 2014.igem.org
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<!--Transformation終わり--> | <!--Transformation終わり--> | ||
| + | |||
| + | <!-- タイトル --> | ||
| + | <h3>June 13, 2014</h3> | ||
| + | <!-- タイトル終わり --> | ||
| + | <!-- 本文開始 --> | ||
| + | <p class="sentence"> | ||
| + | <!-- 実験者名 --> | ||
| + | KOBAYASHI<br> | ||
| + | <!-- 実験者名終わり --> | ||
| + | <!--Colony Isolation--> | ||
| + | <strong>Colony Isolation</strong><br> | ||
| + | Select 6 colonies per plate from 4 plates from June 12th.<br> | ||
| + | <table class="materials"> | ||
| + | <tr> | ||
| + | <th rowspan="2">Host</th> | ||
| + | <th colspan="2">Sample</th> | ||
| + | <th rowspan="2">Section</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Vector</th> | ||
| + | <th>Insert</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>BL21(DE3)pLysS</td> | ||
| + | <td>pGEX6P-2</td> | ||
| + | <td>CuMTSE1</td> | ||
| + | <td>1-a,1-b</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>BL21(DE3)pLysS</td> | ||
| + | <td>pGEX6P-2</td> | ||
| + | <td>CuMTSE2</td> | ||
| + | <td>2-a,2-b</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>BL21(DE3)pLysS</td> | ||
| + | <td>pGEX6P-2</td> | ||
| + | <td>CuMTS3</td> | ||
| + | <td>3-a,3-b</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>BL21(DE3)pLysS</td> | ||
| + | <td>pGEX6P-2</td> | ||
| + | <td>CuMTSE62</td> | ||
| + | <td>6-a,6-b</td> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
| + | <!--Colony Isolation--> | ||
| + | |||
| + | <br> | ||
| + | </p> | ||
| + | <!--本文終わり--> | ||
Revision as of 07:08, 2 October 2014
LabNote
June
June 10, 2014
OKAMOTO
Miniprep
| Host | Sample | |
|---|---|---|
| Vector | Insert | |
| BL21(DE3) | pGEX6P-2 | CuMTSE1 |
| BL21(DE3) | pGEX6P-2 | CuMTSE2 |
| BL21(DE3) | pGEX6P-2 | CuMTS3 |
| BL21(DE3) | pGEX6P-2 | CuMTS62 |
Restriction Digest
| Host | Sample | Enzyme | Buffer | |
|---|---|---|---|---|
| Vector | Insert | |||
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | EcoR1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE1 | Sal1, Not1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE2 | Sal1, Not1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTS3 | Sal1, Not1 | Buffer H |
| BL21(DE3)PlysS | pGEX6P-2 | CuMTSE62 | Sal1, Not1 | Buffer H |
June 11, 2014
KOBAYASHI
AGE
| Lane | Sample/Marker | Enzyme | |
|---|---|---|---|
| Vector | Insert | ||
| 1 | λDNA-HindⅢ | ||
| 2 | pGEX6P-2 | CuMTSE1 | N.D |
| 3 | pGEX6P-2 | CuMTSE1 | EcoR1 |
| 4 | pGEX6P-2 | CuMTSE1 | Sal1,Not1 |
| 5 | pGEX6P-2 | CuMTSE2 | N.D |
| 6 | pGEX6P-2 | CuMTSE2 | EcoR1 |
| 7 | pGEX6P-2 | CuMTSE2 | Sal1, Not1 |
| 8 | pGEX6P-2 | CuMTS3 | N.D |
| 9 | pGEX6P-2 | CuMTS3 | EcoR1 |
| 10 | pGEX6P-2 | CuMTS62 | N.D |
| 11 | pGEX6P-2 | CuMTS62 | EcoR1 |
| 12 | pGEX6P-2 | CuMTS62 | Sal1,Not1 |
June 12, 2014
KOBAYASHI
Transformation
| Host | Sample | |
|---|---|---|
| Vector | Insert | |
| BL21(DE3)pLysS | pGEX6P-2 | CuMTSE1 |
| BL21(DE3)pLysS | pGEX6P-2 | CuMTSE2 |
| BL21(DE3)pLysS | pGEX6P-2 | CuMTS3 |
| BL21(DE3)pLysS | pGEX6P-2 | CuMTS62 |
June 13, 2014
KOBAYASHI
Colony Isolation
Select 6 colonies per plate from 4 plates from June 12th.
| Host | Sample | Section | |
|---|---|---|---|
| Vector | Insert | ||
| BL21(DE3)pLysS | pGEX6P-2 | CuMTSE1 | 1-a,1-b |
| BL21(DE3)pLysS | pGEX6P-2 | CuMTSE2 | 2-a,2-b |
| BL21(DE3)pLysS | pGEX6P-2 | CuMTS3 | 3-a,3-b |
| BL21(DE3)pLysS | pGEX6P-2 | CuMTSE62 | 6-a,6-b |
July
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