Team:KIT-Kyoto/Notebook/Labnote

From 2014.igem.org

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<h3>June 12, 2014</h3>
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<p class="sentence">
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<!-- 実験者名 -->
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KOBAYASHI<br>
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<strong>Transformation</strong>
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<table class="materials">
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<tr>
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<th rowspan="2">Host</th>
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<th colspan="2">Sample</th>
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</tr>
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<tr>
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<th>Vector</th>
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<th>Insert</th>
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</tr>
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<tr>
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<td>BL21(DE3)pLysS</td>
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<td>pGEX6P-2</td>
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<td>CuMTSE1</td>
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</tr>
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<tr>
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<td>BL21(DE3)pLysS</td>
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<td>pGEX6P-2</td>
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<td>CuMTSE2</TD>
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</tr>
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<tr>
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<td>BL21(DE3)pLysS</td>
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<td>pGEX6P-2</td>
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<td>CuMTS3</td>
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<tr>
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<td>BL21(DE3)pLysS</td>
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<td>pGEX6P-2</td>
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<td>CuMTS62</td>
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</tr>
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</table>
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<!--Transformation終わり-->
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Revision as of 06:58, 2 October 2014

LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

June 11, 2014

KOBAYASHI
AGE

Lane Sample/Marker Enzyme
Vector Insert
1 λDNA-HindⅢ
2 pGEX6P-2 CuMTSE1 N.D
3 pGEX6P-2 CuMTSE1 EcoR1
4 pGEX6P-2 CuMTSE1 Sal1,Not1
5 pGEX6P-2 CuMTSE2 N.D
6 pGEX6P-2 CuMTSE2 EcoR1
7 pGEX6P-2 CuMTSE2 Sal1, Not1
8 pGEX6P-2 CuMTS3 N.D
9 pGEX6P-2 CuMTS3 EcoR1
10 pGEX6P-2 CuMTS62 N.D
11 pGEX6P-2 CuMTS62 EcoR1
12 pGEX6P-2 CuMTS62 Sal1,Not1

June 12, 2014

KOBAYASHI
Transformation

Host Sample
Vector Insert
BL21(DE3)pLysS pGEX6P-2 CuMTSE1
BL21(DE3)pLysS pGEX6P-2 CuMTSE2
BL21(DE3)pLysS pGEX6P-2 CuMTS3
BL21(DE3)pLysS pGEX6P-2 CuMTS62

July

7/25



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August




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October




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