Team:Hong Kong HKUST/wetlab/notebook

From 2014.igem.org

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·      Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br>
·      Transformation of pSB1C3-BBa_E0240, pSB1C3-BBa_B0032 and pSB1C3-BBa_K20600<br>
·      Transformation of BBa_B0034 and BBa_E0040 into DH10B<br>
·      Transformation of BBa_B0034 and BBa_E0040 into DH10B<br>
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·      <br>
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<br><b>June 10</b> <br>
<br><b>June 10</b> <br>
Wet lab<br>
Wet lab<br>
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·      Looked for P<sub>combox</sub> gene sequence from the genomic DNA<br>
·      Looked for P<sub>combox</sub> gene sequence from the genomic DNA<br>
·      Bacterial inoculation of BBa_B0034 and BBa_E0040<br>
·      Bacterial inoculation of BBa_B0034 and BBa_E0040<br>
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·   <br>
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<br><b>June 11</b> <br>
<br><b>June 11</b> <br>
Wet lab<br>
Wet lab<br>
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·      Studied the Characteristic, function and mechnism of how promoters work<br>
·      Studied the Characteristic, function and mechnism of how promoters work<br>
·      Miniprep of BBa_B0034 and BBa_E0040<br>
·      Miniprep of BBa_B0034 and BBa_E0040<br>
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·   <br>
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<br><b>June 12</b> <br>
<br><b>June 12</b> <br>
Wet lab<br>
Wet lab<br>
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·      research on gene sequence of PcomX, how to quantify p~<i>comE</i>, range of concentration of  p~<i>comE</i> to use for characterization<br>
·      research on gene sequence of PcomX, how to quantify p~<i>comE</i>, range of concentration of  p~<i>comE</i> to use for characterization<br>
·      explain of scar<br>
·      explain of scar<br>
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·   <br>
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<br><b>June 13</b> <br>
<br><b>June 13</b> <br>
Wet lab<br>
Wet lab<br>
·      Looked for P<sub>combox</sub> gene sequence from the genomic DNA<br>
·      Looked for P<sub>combox</sub> gene sequence from the genomic DNA<br>
·      research on gene sequence of PcomX, how to quantify p~<i>comE</i>, range of concentration of  p~<i>comE</i> to use for characterization<br>
·      research on gene sequence of PcomX, how to quantify p~<i>comE</i>, range of concentration of  p~<i>comE</i> to use for characterization<br>
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·      <br>
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-
·   <br>
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       </div>
       </div>
     </div>
     </div>
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·      Designed plasmid construct for P<sub>combox</sub><br>
·      Designed plasmid construct for P<sub>combox</sub><br>
·      Bacterial inoculation of BBa_J04450(CHL)<br>
·      Bacterial inoculation of BBa_J04450(CHL)<br>
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·   <br>
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<br><b>June 24</b> <br>
<br><b>June 24</b> <br>
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·      Transformation of BBa_J04450(KAN)<br>
·      Transformation of BBa_J04450(KAN)<br>
·      Bacterial inoculation of BBa_J04450(CHL)<br>
·      Bacterial inoculation of BBa_J04450(CHL)<br>
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·      <br>
 
·      <br>
·      <br>
·   <br>
·   <br>
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·      Gel check of purified BBa_B0015 insert<br>
·      Gel check of purified BBa_B0015 insert<br>
·      Transformation of ligated pSB1C3-K880005.B0015<br>
·      Transformation of ligated pSB1C3-K880005.B0015<br>
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·      <i>comE<sup>D58E</sup></i> :Phusion PCR<br>
·      <i>comE<sup>D58E</sup></i> :Phusion PCR<br>
·      E0240: Gel purification<br>
·      E0240: Gel purification<br>
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·      J04450 (KAN): Gel purification<br>
·      J04450 (KAN): Gel purification<br>
·      <i>comE<sup>D58E</sup></i>:Plasmid extraction; NanoDrop; Gel electrophoresis (not <i>comE</i>); Bacterial inoculation<br>
·      <i>comE<sup>D58E</sup></i>:Plasmid extraction; NanoDrop; Gel electrophoresis (not <i>comE</i>); Bacterial inoculation<br>
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·   <br>
 
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·   <br>
 
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·      Extract J04450 (KAN)<br>
·      Extract J04450 (KAN)<br>
·      Restrict check & run mutagenesis <i>comE<sup>D58E</sup></i><br>
·      Restrict check & run mutagenesis <i>comE<sup>D58E</sup></i><br>
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·   <br>
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·   <br>
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<br> <b> July 11</b> <br>
<br> <b> July 11</b> <br>
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·      Gel extract <i>comE<sup>D58E</sup></i><br>
·      Gel extract <i>comE<sup>D58E</sup></i><br>
·      BBa_B0015 Gel purification<br>
·      BBa_B0015 Gel purification<br>
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·      <br>
 
·   <br>
·   <br>
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·      Gel purification of digested pSB3K3 backbone<br>
·      Gel purification of digested pSB3K3 backbone<br>
·      Colony PCR of Promoters<br>
·      Colony PCR of Promoters<br>
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·      Gel electrophoresis of colony PCR results<br>
·      Gel electrophoresis of colony PCR results<br>
·      Mutagenesis of <i>comE<sup>D58E</sup></i><br>
·      Mutagenesis of <i>comE<sup>D58E</sup></i><br>
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·   <br>
 
·   <br>
·   <br>
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·      Received pCEPcin2<br>
·      Received pCEPcin2<br>
·      Gel electrophoresis of colony PCR and mutagenesis results<br>
·      Gel electrophoresis of colony PCR and mutagenesis results<br>
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·   <br>
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·      Gel check on K880005<br>
·      Gel check on K880005<br>
·      Transformation of ligated product (promoters+pSB1C3)<br>
·      Transformation of ligated product (promoters+pSB1C3)<br>
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·      <br>
 
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·      <br>
 
·   <br>
·   <br>
       </div>
       </div>
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·      Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br>
·      Digestion of pSB3K3-BBa_J04450 at XbaI and PstI<br>
·      Restrict check K880005  <br>
·      Restrict check K880005  <br>
 +
<br> <b> July 31</b> <br>
<br> <b> July 31</b> <br>
Wet lab<br>
Wet lab<br>
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·      Bacterial inoculation of PcomX (PcomCDE(3))<br>
·      Bacterial inoculation of PcomX (PcomCDE(3))<br>
·      Gel extraction and purification of <i>comE</i> mutant<br>
·      Gel extraction and purification of <i>comE</i> mutant<br>
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<br><b>Aug 19</b> <br>
<br><b>Aug 19</b> <br>
Wet lab<br>
Wet lab<br>
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·      Digestion of PcomCDE-E0240<br>
·      Digestion of PcomCDE-E0240<br>
·      Colony PCR pSB1C3-PcomCDE, K880005-<i>comE</i> WT, K880005-<i>comE<sup>D58E</sup></i>-myc<br>
·      Colony PCR pSB1C3-PcomCDE, K880005-<i>comE</i> WT, K880005-<i>comE<sup>D58E</sup></i>-myc<br>
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<br><b>Aug 26</b> <br>
<br><b>Aug 26</b> <br>
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·      Ligation w/ <i>comE</i> WT<br>
·      Ligation w/ <i>comE</i> WT<br>
·      Restrict check K880005-<i>comE<sup>D58E</sup></i>-myc<br>
·      Restrict check K880005-<i>comE<sup>D58E</sup></i>-myc<br>
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<br><b>Aug 27</b> <br>
<br><b>Aug 27</b> <br>
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·      PCR check K880005-<i>comE<sup>D58E</sup></i>-myc<br>
·      PCR check K880005-<i>comE<sup>D58E</sup></i>-myc<br>
·      Ligate K880005-<i>comE</i>-myc<br>
·      Ligate K880005-<i>comE</i>-myc<br>
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<br><b>Aug 28</b> <br>
<br><b>Aug 28</b> <br>

Revision as of 14:37, 17 October 2014



Notebook

Throughout the course of our project, we have continuously recorded our daily work, such as wetlab activities and research findings. The notebook for Project Pneuumonsensor and Project Riboregulator are displayed separately.






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