Team:CU-Boulder

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:CU-Boulder&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:CU-Boulder/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=CU-Boulder"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:CU-Boulder/Project"style="color:#000000"> Project</a></td>
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                    <header>
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                        <h2 id="crispr"><span>CRISPR-Cas Mediated Phage Therapy</span></h2>
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                                        <p id="crispr" style="font-size: 18pt; font-weight: bold;">the Future Antibacterial Agent</p>
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                                        <p id="crispr" style="font-size: 18pt; font-style: italic;">University of Colorado at Boulder</p>
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                                                          <a href="http://www.youtube.com/watch?v=NLD6r3_2_a8" title="CU-Boulder Presentation"> 
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  <img src="https://static.igem.org/mediawiki/2014/4/4c/Presentation.gif" alt="CU-Boulder Presentation">
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                        <h2>Issue</h2>
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                    </header>
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                    <p>In the last decade the number of antibiotic resistant bacteria has grown at
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a shocking rate, making these “superbugs” one of our top global health threats.
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This trend indicates that the golden age of antibiotics is ending and makes the
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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search for new antibacterial treatments more important than ever. New bacterial
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<!-- Links to other team pages -->
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treatments need the ability to adapt faster than the harmful bacteria that they
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<ul>
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<li><a href="https://2014.igem.org/Team:CU-Boulder">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:CU-Boulder/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=CU-Boulder">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:CU-Boulder/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:CU-Boulder/Parts">Parts</a> </li>
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<li><a href="https://2014.igem.org/Team:CU-Boulder/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:CU-Boulder/Safety">Safety</a> </li>
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target. Additionally, new treatments must preserve essential bacteria of the
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microbiome and specifically target pathogenic bacteria in a mixed population.</p>
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                <a href="#two" class="button style2 down anchored">Next</a>
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                        <h2>Technology</h2>
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                    <p>New technology has recently emerged that allows us to achieve these goals.
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<td > </td>
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CRISPR-Cas9 consists of an endonuclease (Cas9) that is guided to a specific
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<p>There are a few wiki requirements teams must follow:</p>
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sequence within a genome by a CRISPR RNA component. Once targeted to the  
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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genome, Cas9 causes a double stranded break, killing the host bacterial cell.
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Because the killing relies on the sequence of the CRISPR guide RNA, which can
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be engineered to contain a short specific nucleotide sequence, unique genes
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within any pathogenic bacteria can be targeted.</p>
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                <a href="#three" class="button style2 down anchored">Next</a>
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                        <h2>Our Project</h2>
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                    </header>
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                    <p>Utilizing a non-replicating
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<!--tips  -->
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phage as a vector, we can efectively deliver the CRISPR-Cas9 machinery to cells
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and kill bacteria through CRISPR-Cas9–targeting of the genome. Ongoing
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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experiments are aimed at demonstrating that sequence-specific killing can
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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occur in a mixed population of cells as well as in an in vivo model. We believe
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that this technology, which we refer to as “Pathogen Assassin” will have broad
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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applications ranging from medicine to industry and beyond.</p>
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{{UCB-Footer}}
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and what you plan to do.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Latest revision as of 22:47, 17 October 2014

Big Picture by HTML5 UP

CRISPR-Cas Mediated Phage Therapy

the Future Antibacterial Agent

University of Colorado at Boulder

Issue

In the last decade the number of antibiotic resistant bacteria has grown at a shocking rate, making these “superbugs” one of our top global health threats. This trend indicates that the golden age of antibiotics is ending and makes the search for new antibacterial treatments more important than ever. New bacterial treatments need the ability to adapt faster than the harmful bacteria that they target. Additionally, new treatments must preserve essential bacteria of the microbiome and specifically target pathogenic bacteria in a mixed population.

Next

Technology

New technology has recently emerged that allows us to achieve these goals. CRISPR-Cas9 consists of an endonuclease (Cas9) that is guided to a specific sequence within a genome by a CRISPR RNA component. Once targeted to the genome, Cas9 causes a double stranded break, killing the host bacterial cell. Because the killing relies on the sequence of the CRISPR guide RNA, which can be engineered to contain a short specific nucleotide sequence, unique genes within any pathogenic bacteria can be targeted.

Next

Our Project

Utilizing a non-replicating phage as a vector, we can efectively deliver the CRISPR-Cas9 machinery to cells and kill bacteria through CRISPR-Cas9–targeting of the genome. Ongoing experiments are aimed at demonstrating that sequence-specific killing can occur in a mixed population of cells as well as in an in vivo model. We believe that this technology, which we refer to as “Pathogen Assassin” will have broad applications ranging from medicine to industry and beyond.


Retrieved from "http://2014.igem.org/Team:CU-Boulder"