Team:EPF Lausanne

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            <li><a href="https://2014.igem.org/Team:EPF_Lausanne/Envelope_stress_responsive_bacteria">Stress Responsive Bacteria</a></li>
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<figcaption class="cntr">Association of split IFP 1.4 fragments</figcaption></div>
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The 2014 EPFL iGEM team has been working on showing that biologically engineered organisms can detect and process signals quickly and efficiently. With this in mind, our team brought forward a novel idea: combining Protein Complementation techniques with biosensors to achieve fast spatiotemporal analysis of cell response to stimuli.  
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The 2014 EPFL iGEM team has been working on showing that biologically engineered organisms can detect and process signals quickly and efficiently. With this in mind, our team brought forward a novel idea: combining protein complementation techniques with biosensors to achieve fast spatiotemporal analysis of cell responses to stimuli. In other words, we fused complementary reporter protein fragments to interacting proteins. The presence of a given stimulus leads to the interaction of the proteins of interest thus allowing the fused split complements to re-acquire their functional conformation and emit signal. We thereby are able to detect signal dynamics by relying on much faster post-transcriptional modifications rather than slow traditional reporter transcription.
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we are able to detect signal dynamics thanks to the association of the reporter fragments. We thereby rely on much faster post-transcriptional modifications to generate signals rather than traditional reporter transcription.
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The principle is the following: two complementary fragments of a reporter protein are fused to interacting proteins. When the interaction is stimulated, the two fragments associate, thereby reconstituting the reporter signal in a much faster way than traditional post-transcriptional reporters.
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As a proof-of-concept, we aimed to develop the first BioPad: a biological TouchPad made of touch-responsive bacteria in a microfluidic chip allowing the control of electronic devices. This was achieved by engineering two stress related pathways in E.Coli and S.Cerevisiae. In E.Coli, we engineered the Cpx Pathway – a two-component regulatory system responsive to envelope stress. In S.Cerevisiae we modified the HOG Pathway - a MAPKK pathway responsive to osmotic stress. To learn more about our the various components of our project, check out our <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Overview">overview section</a>. If you are a judge, you might also be interested in our <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Data">data page</a>.</p>
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As a proof-of-concept, we aimed to develop the first BioPad: a biological trackpad made of a microfluidic chip, touch-responsive organisms and a signal detector. To make our organisms touch-sensitive, we engineering two stress-related pathways in <i>E. coli</i> and <i>S. cerevisiae</i>.<!--In <i>E. coli</i>, we engineered the Cpx Pathway - a two-component regulatory system responsive to envelope stress. In <i>S. cerevisiae</i>, we modified the HOG Pathway - a MAPKK pathway responsive to osmotic stress.--> As for the reporter proteins, we worked mainly with fluorescent proteins but also implemented a split luciferase complementation assay. To learn more about the various components of our project, check out our <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Overview">overview section</a>, as well as the different <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Parts">parts</a> submitted by our team. If you are a judge, you might also be interested in our <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Results">results page</a>, our <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Data">data page</a> and our <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Judging">judging form</a>. </p>
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As for applied sciences, the BioPad could be used to deliver a cheap, fast, efficient, and accurate antibiotic screening system allowing researchers to easily quantify the effects of antibiotics on gram-negative bacteria. The BioPad project could also be the source of an "antibiotic complement" drug increasing the efficiency of pre-existing antibiotics. Moreover, the Biopad could provide a new approach to studying genes by allowing researchers to examine the relationship between genes and their corresponding activating signals. To learn more about the applications of our project click <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Applications">here</a>.</p>
As for applied sciences, the BioPad could be used to deliver a cheap, fast, efficient, and accurate antibiotic screening system allowing researchers to easily quantify the effects of antibiotics on gram-negative bacteria. The BioPad project could also be the source of an "antibiotic complement" drug increasing the efficiency of pre-existing antibiotics. Moreover, the Biopad could provide a new approach to studying genes by allowing researchers to examine the relationship between genes and their corresponding activating signals. To learn more about the applications of our project click <a target="_blank" href="https://2014.igem.org/Team:EPF_Lausanne/Applications">here</a>.</p>
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         <h1>Stress responsive bacteria </h1>
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   <p class="lead">Can't touch this</p>
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   <p class="lead">Find out how we took advantage of the Cpx pathway and split IFP1.4 to give birth to bacteria emitting fast signals in response to chemical and mechanical stresses!</p>
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         <h1>Osmo responsive yeast</h1>
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   <p class="lead">Our Biopad is implemented in a microfluidic chip. This tool allows all kinds of analytical experiments
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   <p class="lead">Discover how we engineered the HOG osmotic response pathway to create touch sensitive yeast strains! Learn more on how we implemented a split GFP and a split Luciferase in <i>S. cerevisiae</i> leading to light emission when pressure is applied.</p>
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  and is increasingly used in biological research. From fabrication to applications, find out more about
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  this awesome device here!</p>
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   <p class="lead">Discover how we took advantage of the HOG osmotic response pathway to create touch sensitive yeast strains! Learn more on how we implemented a split GFP and a split Luciferase in S.Cerevisiae leading to light emission when pressure is applied.</p>
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   <p class="lead">Our Biopad is implemented in a microfluidic chip. This tool allows all kinds of analytical experiments
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  and is increasingly used in biological research. From fabrication to applications, find out more about
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  this awesome device here!</p>
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   <p class="lead">In order to process our data quickly and automatically, we built an interface with a Raspberry Pi and a camera. Discover how <br />it works by clicking here!</p>
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   <p class="lead">Are we human, or are we dancers ?</p>
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   <p class="lead">Our project is well suited to show the general public the power of synthetic biology. Find out how we introduced this domain to the younger generation, and how they developed their own mini iGEM projects to tackle everyday problems with enthusiasm and creativity.</p>
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<div class="span6"><h1 class="cntr">MEET OUR TEAM</h1>
<div class="span6"><h1 class="cntr">MEET OUR TEAM</h1>
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<p>We are a group of 14 students from the faculties of Life, Biomechanical, and Computer Sciences, and are supervised by 2 EPFL professors, 1 Lecturer and 5 PhD students.</p></div>
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<p>We are a group of 13 students from the faculties of Life Sciences & Technologies and Computer Sciences, </br>and are supervised by 2 EPFL professors, 1 Lecturer and 5 PhD students.</p></div>
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Latest revision as of 03:50, 18 October 2014

Our project in a nutshell




EPFL_interaction_IFP_cartoon
Association of split IFP 1.4 fragments

The 2014 EPFL iGEM team has been working on showing that biologically engineered organisms can detect and process signals quickly and efficiently. With this in mind, our team brought forward a novel idea: combining protein complementation techniques with biosensors to achieve fast spatiotemporal analysis of cell responses to stimuli. In other words, we fused complementary reporter protein fragments to interacting proteins. The presence of a given stimulus leads to the interaction of the proteins of interest thus allowing the fused split complements to re-acquire their functional conformation and emit signal. We thereby are able to detect signal dynamics by relying on much faster post-transcriptional modifications rather than slow traditional reporter transcription.

As a proof-of-concept, we aimed to develop the first BioPad: a biological trackpad made of a microfluidic chip, touch-responsive organisms and a signal detector. To make our organisms touch-sensitive, we engineering two stress-related pathways in E. coli and S. cerevisiae. As for the reporter proteins, we worked mainly with fluorescent proteins but also implemented a split luciferase complementation assay. To learn more about the various components of our project, check out our overview section, as well as the different parts submitted by our team. If you are a judge, you might also be interested in our results page, our data page and our judging form.

MEET OUR TEAM

We are a group of 13 students from the faculties of Life Sciences & Technologies and Computer Sciences,
and are supervised by 2 EPFL professors, 1 Lecturer and 5 PhD students.

the team's students

Sponsors