Team:Aachen/Project/2D Biosensor
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{{Team:Aachen/FigureFloat|Aachen_Chip_medium_geldoc.png|title=Differend medium in the Gel Doc™|subtitle=complex media (LB) exhibited high background fluorescence while less background fluorescence was observed with the minimal media (HM, M9, NA).|right|width=500px}} | {{Team:Aachen/FigureFloat|Aachen_Chip_medium_geldoc.png|title=Differend medium in the Gel Doc™|subtitle=complex media (LB) exhibited high background fluorescence while less background fluorescence was observed with the minimal media (HM, M9, NA).|right|width=500px}} | ||
{{Team:Aachen/FigureFloat|Aachen_5days_K131026_neb_tb_1,5h.jpg |title=Testing our chips' shelf-life.|subtitle= [http://parts.igem.org/Part:BBa_K131026 K131026] in NEB induced after 5 days of storage at 4 °C. The right chip was induced with 0.2 µL of 500 µg/mL HSL, and the image was taken after 1.5 h.|left|width=500px}} | {{Team:Aachen/FigureFloat|Aachen_5days_K131026_neb_tb_1,5h.jpg |title=Testing our chips' shelf-life.|subtitle= [http://parts.igem.org/Part:BBa_K131026 K131026] in NEB induced after 5 days of storage at 4 °C. The right chip was induced with 0.2 µL of 500 µg/mL HSL, and the image was taken after 1.5 h.|left|width=500px}} | ||
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To determine which medium enables fast and reliable fluorescence response detection of our chip system, the growth behavior on as well as the fluorescence properties of several media have been investigated. The complex media LB, TB and NA and the minimal media M9 and HM were tested. | To determine which medium enables fast and reliable fluorescence response detection of our chip system, the growth behavior on as well as the fluorescence properties of several media have been investigated. The complex media LB, TB and NA and the minimal media M9 and HM were tested. | ||
Because of the reduced background fluorescence compared to TB medium when using Watson for sensor chip evaluation and because of sufficient cultivation conditions for our 'Cellocks, LB medium was chosen for sensor chip manufacturing (table below). | Because of the reduced background fluorescence compared to TB medium when using Watson for sensor chip evaluation and because of sufficient cultivation conditions for our 'Cellocks, LB medium was chosen for sensor chip manufacturing (table below). | ||
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Further experiments were conducted to test long-time storage of the sensor chips. Storage at -20 °C resulted in the loss of our sensor cells. Adding 5-10% (v/v) glycerol ensured survival of the sensor cells, but resulted in an expression stop of fluorescence proteins. Thus, the idea of long time storage of the sensor chips had to be passed on. However, it was possible to store ready-to-use sensor chips for 2 days at 4 °C when using LB medium and storage for 5 days was possible with chips made from TB medium. | Further experiments were conducted to test long-time storage of the sensor chips. Storage at -20 °C resulted in the loss of our sensor cells. Adding 5-10% (v/v) glycerol ensured survival of the sensor cells, but resulted in an expression stop of fluorescence proteins. Thus, the idea of long time storage of the sensor chips had to be passed on. However, it was possible to store ready-to-use sensor chips for 2 days at 4 °C when using LB medium and storage for 5 days was possible with chips made from TB medium. |
Revision as of 14:40, 17 October 2014
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