Team:Aachen/Project/2D Biosensor
From 2014.igem.org
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=== Induction === | === Induction === | ||
For artificially induction of our molecular detection constructs we simluated the presence of ''P. aeruginosa'' by use of IPTG or 3-oxo-C12 HSL. | For artificially induction of our molecular detection constructs we simluated the presence of ''P. aeruginosa'' by use of IPTG or 3-oxo-C12 HSL. | ||
- | A minimal volume was desired for induction, because initial experiments showed that diffusion of the inducers through the chip hindered detection of distinct spots on the chips. The lower boundary of the volume we could pipett was limitted to 0.2 µL due to the pipetts available. | + | A minimal pipetting volume was desired for induction, because initial experiments showed that diffusion of the inducers through the chip hindered detection of distinct spots on the chips. The lower boundary of the volume we could pipett was limitted to 0.2 µL due to the pipetts available. |
Sensor cells based on ''E. coli'' BL21 cells, which incorporated the [https://2014.igem.org/Team:Aachen/Parts#partsK1319042 K1319042] construct were able to detect IPTG concentrations down to 1 mM (0.2 µL), so were sensor cells based on ''E. coli'' BL21 cells, which incorporated the REACh constructs. | Sensor cells based on ''E. coli'' BL21 cells, which incorporated the [https://2014.igem.org/Team:Aachen/Parts#partsK1319042 K1319042] construct were able to detect IPTG concentrations down to 1 mM (0.2 µL), so were sensor cells based on ''E. coli'' BL21 cells, which incorporated the REACh constructs. | ||
Sensor cells based on ''E. coli'' BL21 cells, which incorporated the [http://parts.igem.org/Part:BBa_K131026 K131026] construct were able to detect HSL concentrations down to 500 µg/mL (0.2 µL). Further more, detection of growing ''Pseudomonas aeruginosa'' cells based on secreted HSLs was possible using the [http://parts.igem.org/Part:BBa_K131026 K131026] construct. A detailed description incluing pictures of the experiments leading to the findings mentioned above can be found in the [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements Achievements] section. | Sensor cells based on ''E. coli'' BL21 cells, which incorporated the [http://parts.igem.org/Part:BBa_K131026 K131026] construct were able to detect HSL concentrations down to 500 µg/mL (0.2 µL). Further more, detection of growing ''Pseudomonas aeruginosa'' cells based on secreted HSLs was possible using the [http://parts.igem.org/Part:BBa_K131026 K131026] construct. A detailed description incluing pictures of the experiments leading to the findings mentioned above can be found in the [https://2014.igem.org/Team:Aachen/Project/2D_Biosensor#biosensorachievements Achievements] section. |
Revision as of 14:31, 16 October 2014
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