Team:Aachen/Project/2D Biosensor
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[[File:Aachen_14-10-14_cellock_stehend_panel.png|right|150px]] | [[File:Aachen_14-10-14_cellock_stehend_panel.png|right|150px]] | ||
- | == | + | === Results === |
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We are able to detect IPTG, 3-oxo-C{{sub|12}} HSL and ''Pseudomonas aeruginosa''. To proof that your sensor cells produce the flourescence signal and not something else we have B0015 in NEB as negativ control. | We are able to detect IPTG, 3-oxo-C{{sub|12}} HSL and ''Pseudomonas aeruginosa''. To proof that your sensor cells produce the flourescence signal and not something else we have B0015 in NEB as negativ control. | ||
- | === Testing our Sensor Chips with a Platereader === | + | {{Team:Aachen/Figure|Aachen_B0015_IPTG_HSL_Pseudomonas.png|title=Negativ control.|B0015 in NEB as negativ control induced with A) 0.2 µl of 100 mM IPTG, image taken after 2.5 h B) 0.2 µl of 500 µg/ml HSL (3-oxo-C12), image after 2.5 h |width=900px}} C) with 5 spots of ''Pseudomonas aeruginosa'' on the left and one big spot on the right, image taken after 2h |
+ | |||
+ | ==== Testing our Sensor Chips with a Platereader ==== | ||
{{Team:Aachen/Figure|Aachen_K1319042_Platereader.gif|title=Testing K1319042 in our sensor chips|subtitle=K1319042 in our sensorchip induced with 2 µl iPTG and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | {{Team:Aachen/Figure|Aachen_K1319042_Platereader.gif|title=Testing K1319042 in our sensor chips|subtitle=K1319042 in our sensorchip induced with 2 µl iPTG and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | ||
{{Team:Aachen/Figure|Aachen_K131026_Platereader.gif|title=Testing K131026 in our sensor chips|subtitle=K131026 in our sensorchip induced with 0.2 µl 3-oxo-C{{sub|12}} HSL and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | {{Team:Aachen/Figure|Aachen_K131026_Platereader.gif|title=Testing K131026 in our sensor chips|subtitle=K131026 in our sensorchip induced with 0.2 µl 3-oxo-C{{sub|12}} HSL and measured with a Platereader. Blue color indicates no fluorescence, Red color indicates fluorescence.|width=300px}} | ||
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{{Team:Aachen/Figure|Aachen_K131026_HSLdetection_slow.gif|title=Detection of 3-oxo-C{{sub|12}} HSL with K131026|subtitle=0,2 µl of 3-oxo-C{{sub|12}} HSL were put in the middle of the chip and then incubated at 37 °C in our WatsOn device.|width=480px}} | {{Team:Aachen/Figure|Aachen_K131026_HSLdetection_slow.gif|title=Detection of 3-oxo-C{{sub|12}} HSL with K131026|subtitle=0,2 µl of 3-oxo-C{{sub|12}} HSL were put in the middle of the chip and then incubated at 37 °C in our WatsOn device.|width=480px}} | ||
</center> | </center> | ||
- | Detecting Pseudomonas | + | Detecting Pseudomonas aeruginosa with K131026 in our sensor chip with WatsOn. |
- | {{Team:Aachen/Figure|Aachen_K131026_Pseudomonas_aeruginosa_detection.gif|title=Detection of ''Pseudomonas | + | {{Team:Aachen/Figure|Aachen_K131026_Pseudomonas_aeruginosa_detection.gif|title=Detection of ''Pseudomonas aeruginosa'' with K131026|subtitle=Direct detection of ''Pseudomonas aeruginosa on our sensor chips. Sensor cell used were K131026.|width=480px}} |
{{Team:Aachen/Footer}} | {{Team:Aachen/Footer}} |
Revision as of 08:33, 16 October 2014
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