Team:Saarland/Test
From 2014.igem.org
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<img src="https://static.igem.org/mediawiki/2014/6/6c/Pfeil_01.png"> | <img src="https://static.igem.org/mediawiki/2014/6/6c/Pfeil_01.png"> | ||
<p> 30 cycles</p> | <p> 30 cycles</p> |
Revision as of 14:16, 15 October 2014
The following speed cycle program was used:
Step | Temperature [°C] | Time [s] |
---|---|---|
1. Initial Denaturation | 98 | 30 |
2. Denaturation | 98 | 15 |
3. Annealing | 60 | 15 |
4. Extention | 75 | 20/kb |
5. Final Extention | 72 | 120 |
6. storage | 4 | for ever |
30 cycles
Impressum/Copyright