Team:Aachen/Project
From 2014.igem.org
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- | As a '''proof-of-concept''' for ''Cellock Holmes'' we decided to detect the bacterium '''''Pseudomonas aeruginosa'''''. This opportunistic bacterium mainly infects patients with open wounds and burns as well as | + | As a '''proof-of-concept''' for ''Cellock Holmes'' we decided to detect the bacterium '''''Pseudomonas aeruginosa'''''. This opportunistic bacterium mainly infects patients with open wounds and burns as well as immunodeficient people. ''P. aeruginosa'' cells use quorum sensing to communicate with each other, and in doing so secrete autoinducers into their environment. Using Synthetic Biology, our team '''engineered sensor cells''', so-called '''''Cellocks''''', that are able to detect said autoinducers and to elicit a fluorescence signal. Furthermore, the response time of our sensor cells is sped up, through the use of our special [https://2014.igem.org/Team:Aachen/Project/FRET_Reporter '''REACh construct''']. |
So far ''Cellocks'' are able to detect ''P. aeruginosa'' only, however, due to the modular composition of our genetic device, it is possible to engineer ''Cellocks'' in a way that they are able to detect other bacteria's autoinducers. Even more flexibility would be introduced using our [https://2014.igem.org/Team:Aachen/Project/Gal3 '''alternative molecular approach using Galectin-3''']. | So far ''Cellocks'' are able to detect ''P. aeruginosa'' only, however, due to the modular composition of our genetic device, it is possible to engineer ''Cellocks'' in a way that they are able to detect other bacteria's autoinducers. Even more flexibility would be introduced using our [https://2014.igem.org/Team:Aachen/Project/Gal3 '''alternative molecular approach using Galectin-3''']. |
Revision as of 16:53, 14 October 2014
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