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Team:Evry/Interlab Study/09-19-2014
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<h2>Construction n°1: PSB1C3 with I20260</h2> | <h2>Construction n°1: PSB1C3 with I20260</h2> | ||
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| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/7/79/Fluorescence_according_to_OD_for_the_construction_BBa_I20260_in_PSB1C3.jpg" width="600px;" class="thumbimage"/> | ||
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| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/4/44/GFP_concentration_according_to_OD_for_the_construction_I20260_in_PSB1C3.jpg" width="600px;" class="thumbimage"/> | ||
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| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/a/a9/Normalized_GFP_concentration_according_to_time_for_the_I20260_construction_in_PSB1C3.jpg" width="600px;" class="thumbimage"/> | ||
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| + | <a href="https://static.igem.org/mediawiki/2014/a/a9/Normalized_GFP_concentration_according_to_time_for_the_I20260_construction_in_PSB1C3.jpg" class="internal" title="Enlarge"> | ||
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | <br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> | ||
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| + | Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | <li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
| - | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200.<br> | + | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).<br> |
| - | < | + | <FONT color="red">OVERFLOW</FONT> <br> |
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<i>PSB1C3+E0240+J23104</i>: <br> | <i>PSB1C3+E0240+J23104</i>: <br> | ||
| - | Measure of the cells' growth and fluorecence on TECAN infiniteM200 ( | + | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h30-8h30).<br> |
<img src="https://static.igem.org/mediawiki/2014/8/8e/TECAN104.jpg" alt="PLAN DE PLAQUE" /> | <img src="https://static.igem.org/mediawiki/2014/8/8e/TECAN104.jpg" alt="PLAN DE PLAQUE" /> | ||
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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | <li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
| - | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200.<br> | + | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).<br> |
| - | <img src="" alt=" | + | <img src="https://static.igem.org/mediawiki/2014/e/ed/J23105_19.09.png" alt="Plaque" /> |
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| + | <i>PSB1C3+E0240+J23107</i>: <br> | ||
| + | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
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<i>PSB1C3+E0240+J23115</i>: <br> | <i>PSB1C3+E0240+J23115</i>: <br> | ||
| - | Measure of the cells' growth and fluorecence on TECAN infiniteM200 ( | + | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (10h50-17h50).<br> |
| - | <img src="" alt="IMAGE DU PLAN DE PLAQUE" /> | + | <img src="https://static.igem.org/mediawiki/2014/4/44/J23115_19.09.png" alt="IMAGE DU PLAN DE PLAQUE" /> |
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<i>PSB1C3+E0240+J23116</i>: <br> | <i>PSB1C3+E0240+J23116</i>: <br> | ||
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).<br> | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).<br> | ||
| - | <img src="" alt="IMAGE DU PLAN DE PLAQUE" /> | + | <img src="https://static.igem.org/mediawiki/2014/d/d9/J23116_19.09.png" alt="IMAGE DU PLAN DE PLAQUE" /> |
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| - | + | <i>PSB1C3+E0240+J23118</i>: <br> | |
| + | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
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<span class="cd-date">Sep 19</span> | <span class="cd-date">Sep 19</span> | ||
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Latest revision as of 14:48, 8 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23100:
- Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
- Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
OVERFLOW
PSB1C3+E0240+J23104:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h30-8h30).
PSB1C3+E0240+J23105:
- Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
- Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
PSB1C3+E0240+J23107:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (10h50-17h50).
PSB1C3+E0240+J23116:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).
PSB1C3+E0240+J23117:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h00-8h00).
PSB1C3+E0240+J23118:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Sep 19
