Team:Evry/Interlab Study/09-19-2014

From 2014.igem.org

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
    OVERFLOW

PSB1C3+E0240+J23104:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h30-8h30).
PLAN DE PLAQUE

PSB1C3+E0240+J23105:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
    Plaque

PSB1C3+E0240+J23107:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (10h50-17h50).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23116:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23117:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h00-8h00).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23118:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Sep 19