Team:Evry/Interlab Study/09-19-2014

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<h2>Construction n°1: PSB1C3 with I20260</h2>
<h2>Construction n°1: PSB1C3 with I20260</h2>
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
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Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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<i>PSB1C3+E0240+J23105</i>:  
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<i>PSB1C3+E0240+J23100</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200.<br>
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<img src="localisation of the image" alt="IMAGE DU PLAN DE PLAQUE" />
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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).<br>
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<FONT color="red">OVERFLOW</FONT> <br>
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<i>PSB1C3+E0240+J23104</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h30-8h30).<br>
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<img src="https://static.igem.org/mediawiki/2014/8/8e/TECAN104.jpg" alt="PLAN DE PLAQUE" />
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<i>PSB1C3+E0240+J23105</i>: <br>
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<ul>
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<li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).<br>
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<img src="https://static.igem.org/mediawiki/2014/e/ed/J23105_19.09.png" alt="Plaque" />
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<i>PSB1C3+E0240+J23107</i>: <br>
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Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<i>PSB1C3+E0240+J23115</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 (10h50-17h50).<br>
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<img src="https://static.igem.org/mediawiki/2014/4/44/J23115_19.09.png" alt="IMAGE DU PLAN DE PLAQUE" />
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<i>PSB1C3+E0240+J23116</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).<br>
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<img src="https://static.igem.org/mediawiki/2014/d/d9/J23116_19.09.png" alt="IMAGE DU PLAN DE PLAQUE" />
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<i>PSB1C3+E0240+J23117</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h00-8h00).<br>
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<img src="https://static.igem.org/mediawiki/2014/2/2b/TECAN117.jpg" alt="IMAGE DU PLAN DE PLAQUE" />
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<i>PSB1C3+E0240+J23118</i>: <br>
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Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<span class="cd-date">Sep 19</span>  
<span class="cd-date">Sep 19</span>  
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Latest revision as of 14:48, 8 October 2014

Picture

Construction n°1: PSB1C3 with I20260


IMAGE
Symbol
IMAGE
Symbol
IMAGE
Symbol


Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
    OVERFLOW

PSB1C3+E0240+J23104:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h30-8h30).
PLAN DE PLAQUE

PSB1C3+E0240+J23105:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
    Plaque

PSB1C3+E0240+J23107:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (10h50-17h50).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23116:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23117:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h00-8h00).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23118:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Sep 19

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