Team:Evry/Interlab Study/09-19-2014

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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>
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Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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Revision as of 21:01, 19 September 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h-18h at 37°C.

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
    IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23104:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h00-8h00).
PLAN DE PLAQUE

PSB1C3+E0240+J23105:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (18h30-2h30).
    IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23107:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23115:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23116:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (11h00-18h00).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23117:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (1h00-8h00).
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23118:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.


Sep 19