Team:SUSTC-Shenzhen/Notebook/A-B Toxin/Nickel Column Recovery

From 2014.igem.org

Team SUSTC-Shenzhen

Notebook

Element of an endeavor

Contents


A-B Toxin

2014.10.6 to recover the nickel column

protocol

recovery buffer: 0.2M HAc, 8M Urea, PH 4.0

  1. 2CV recovery buffer
  2. 5CV ddH2O
  3. 3CV 2% SDS
    3CV ddH2O
  4. 1CV 25%ethanol
  5. 1CV 50%ethanol
  6. 1CV 75%ethanol
  7. 1CV 100%ethanol
  8. 1CV 75%ethanol
  9. 1CV 50%ethanol
  10. 1CV 25%ethanol
  11. 3CV ddH2O
  12. 5CV 0.1M EDTA
  13. 5CV ddH2O
  14. 5CV 0.1M NiCl2
  15. 3CV ddH2O
  16. 2CV revovery buffer
  17. 2CV loading buffer

Maintained by the iGEM team SUSTC-Shenzhen.

Licensed under CC BY 4.0.

  • ©2014 SUSTC-Shenzhen

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