Golden Gate Universal Transposon Plasmid

A PCR on 63 white colonies in the plate of trasformed cells is realized. Two colonies of 63 have the band expected.
the product of these PCR is purified.

Verification of insert and Isolation of Transposon Plasmid

The Universal Transposon Plasmid is purified.
After that this purification is digest with Bgl II to isolated the Transposon Plasmid of pSB1C3.

Oct 09